CAJ | 학술논문

背景：静电纺技术制备的生物支架能够模拟细胞外基质的纳米纤维结构，因此在再生医学和组织工程领域受到了普遍关注。目的：回顾近年来在增加静电纺纳米纤维支架孔隙率，增大孔隙直径，促进细胞渗透的相关技术，以期发现最具有实用性和经济性的支架制作工艺。方法：由第一作者检索CNKI全文数据库、万方数据库及PubMed数据库2004年1月至2014年10月的相关文献，检索关键词为“细胞渗透，静电纺，三维支架；cel infiltration，3D scaffold，electrospinning”。结果与结论：静电纺技术是目前制备纳米纤维支架的最有效方法，使用静电纺支架作为组织工程支架的基础研究逐年增加。然而，纳米纤维支架内部的纳米级孔隙使细胞只能局限于支架表面生长，因此近年来的研究热点已从制备二维支架向具有疏松多孔结构，能够促进细胞渗透生长的三维支架转换。从简单的调整电纺速率，改变电纺原料简易方法到需要各种复杂设备的方法已被应用于该领域的研究，但现有研究方法仍不成熟稳定，并且多数只应用于体外细胞植入或小动物皮下植入后观察研究，尚未将以上各类方法应用于具体器官的组织工程修复，仍需要进一步的长期比较性研究以证实各类方法的可行性。
배경：정전방기술제비적생물지가능구모의세포외기질적납미섬유결구，인차재재생의학화조직공정영역수도료보편관주。목적：회고근년래재증가정전방납미섬유지가공극솔，증대공극직경，촉진세포삼투적상관기술，이기발현최구유실용성화경제성적지가제작공예。방법：유제일작자검색CNKI전문수거고、만방수거고급PubMed수거고2004년1월지2014년10월적상관문헌，검색관건사위“세포삼투，정전방，삼유지가；cel infiltration，3D scaffold，electrospinning”。결과여결론：정전방기술시목전제비납미섬유지가적최유효방법，사용정전방지가작위조직공정지가적기출연구축년증가。연이，납미섬유지가내부적납미급공극사세포지능국한우지가표면생장，인차근년래적연구열점이종제비이유지가향구유소송다공결구，능구촉진세포삼투생장적삼유지가전환。종간단적조정전방속솔，개변전방원료간역방법도수요각충복잡설비적방법이피응용우해영역적연구，단현유연구방법잉불성숙은정，병차다수지응용우체외세포식입혹소동물피하식입후관찰연구，상미장이상각류방법응용우구체기관적조직공정수복，잉수요진일보적장기비교성연구이증실각류방법적가행성。
BACKGROUND:The electrospinning technique has been used to prepare biological scaffolds to simulate nano-fiber structure of extracelular matrix; therefore, widespread attention has been paid to the electrospinning technique in the field of regenerative medicine and tissue engineering. OBJECTIVE: To review the articles about increasing electrospun nanofiber scaffold porosity, enlarging pore diameter, promoting cel infiltration with related technologies, in order to discover the most practical and economical technology. METHODS:The first author retrieved CNKI database, Wanfang database and PubMed with the keywords of “cel infiltration, 3D scaffold, electrospinning” in Chinese and English, respectively. Literature retrieval period was from January 2004 to October 2014. RESULTS AND CONCLUSION:Electrospinning technology is the most effective method for preparation of nanofiber scaffolds. Electrospinning scaffolds as tissue engineering scaffolds have become an issue of concern in the basic research year by year. However, the internal nano-scale pore of nanofiber scaffolds limits the cels to grow on the surface, so recent research has been focused on highly porous three-dimensional structure which can promote the permeable growth of cels instead of two-dimensional scaffolds. Several techniques have been used, which go from the adjustment of materials and speed of electrospinning to the applications of various kinds of complicated machines. However, the existing researches are stil not mature and stable, the majority of which are applied onlyin vitro as cel implantation or subcutaneous implantation in smal animals. The above-mentioned methods stil need long-term comparative studies to confirm the feasibility in the tissue-engineered repair of organs.