CAJ | 학술논문

生物材料%材料相容性%纤维纳米支架材料%静电纺丝%聚乳酸聚乙醇酸%聚乙二醇%复合支架%生物相容性%小鼠神经干细胞%细胞毒性%细胞增殖%细胞黏附率%国家自然科学基金生物材料%材料相容性%纖維納米支架材料%靜電紡絲%聚乳痠聚乙醇痠%聚乙二醇%複閤支架%生物相容性%小鼠神經榦細胞%細胞毒性%細胞增殖%細胞黏附率%國傢自然科學基金
생물재료%재료상용성%섬유납미지가재료%정전방사%취유산취을순산%취을이순%복합지가%생물상용성%소서신경간세포%세포독성%세포증식%세포점부솔%국가자연과학기금
biocompatible materials%neural stem cels%mice%cel proliferation

背景：聚乳酸聚乙醇酸支架材料广泛应用于组织工程学领域，但其细胞黏附性较差、缺乏活性功能基团以及疏水性较强等缺点限制了其进一步的发展和应用。目的：观察小鼠神经干细胞与静电纺丝聚乳酸聚乙醇酸/聚乙二醇共聚物纳米纤维支架材料的体外相容性。方法：自孕15 d CD-1小鼠胚胎大脑皮质分离培养小鼠神经干细胞。静电纺丝法制备聚乳酸聚乙醇酸和聚乳酸聚乙醇酸/聚乙二醇纳米纤维支架材料，扫描电镜观察材料结构；取第5代神经干细胞分别接种于聚乳酸聚乙醇酸和静电纺丝聚乳酸聚乙醇酸/聚乙二醇纳米纤维支架材料上，进行体外培养。结果与结论：扫描电镜检测显示，两种支架材料呈现相互交联的多孔网状结构。聚乳酸聚乙醇酸组和静电纺丝聚乳酸聚乙醇酸/聚乙二醇组纤维直径和孔隙率差异无显著性意义(P >0.05)。CCK-8检测显示，两种材料无明显细胞毒性。神经干细胞在支架材料中生长良好，两组吸光度值均随培养时间延长而增大，两组在培养1，3，5，7，9，11 d吸光度值差异均有显著性意义(P <0.05)。两组材料培养3，6，9 h，静电纺丝聚乳酸聚乙醇酸/聚乙二醇组的细胞黏附率明显高于聚乳酸聚乙醇酸组(P <0.05)。Hoechst染色显示两组细胞核质均染，形态正常，静电纺丝聚乳酸聚乙醇酸/聚乙二醇组细胞数量明显多于聚乳酸聚乙醇酸组(P <0.05)。扫描电镜观察显示，与聚乳酸聚乙醇酸组相比，静电纺丝聚乳酸聚乙醇酸/聚乙二醇组神经干细胞在支架上的生长情况和基质分泌更好。结果说明，静电纺丝法制备的静电纺丝聚乳酸聚乙醇酸/聚乙二醇纳米纤维支架细胞生物相容性良好，安全无毒，具备合适的孔径和孔隙率，适宜神经干细胞生长，是一种适用于组织工程优质的支架载体。
배경：취유산취을순산지가재료엄범응용우조직공정학영역，단기세포점부성교차、결핍활성공능기단이급소수성교강등결점한제료기진일보적발전화응용。목적：관찰소서신경간세포여정전방사취유산취을순산/취을이순공취물납미섬유지가재료적체외상용성。방법：자잉15 d CD-1소서배태대뇌피질분리배양소서신경간세포。정전방사법제비취유산취을순산화취유산취을순산/취을이순납미섬유지가재료，소묘전경관찰재료결구；취제5대신경간세포분별접충우취유산취을순산화정전방사취유산취을순산/취을이순납미섬유지가재료상，진행체외배양。결과여결론：소묘전경검측현시，량충지가재료정현상호교련적다공망상결구。취유산취을순산조화정전방사취유산취을순산/취을이순조섬유직경화공극솔차이무현저성의의(P >0.05)。CCK-8검측현시，량충재료무명현세포독성。신경간세포재지가재료중생장량호，량조흡광도치균수배양시간연장이증대，량조재배양1，3，5，7，9，11 d흡광도치차이균유현저성의의(P <0.05)。량조재료배양3，6，9 h，정전방사취유산취을순산/취을이순조적세포점부솔명현고우취유산취을순산조(P <0.05)。Hoechst염색현시량조세포핵질균염，형태정상，정전방사취유산취을순산/취을이순조세포수량명현다우취유산취을순산조(P <0.05)。소묘전경관찰현시，여취유산취을순산조상비，정전방사취유산취을순산/취을이순조신경간세포재지가상적생장정황화기질분비경호。결과설명，정전방사법제비적정전방사취유산취을순산/취을이순납미섬유지가세포생물상용성량호，안전무독，구비합괄적공경화공극솔，괄의신경간세포생장，시일충괄용우조직공정우질적지가재체。
BACKGROUND:Poly(lactide-co-glycolide) (PLGA) scaffold is widely used in tissue engineering, but its poor cel adhesion ability and strong hydrophobicity limit its further development and application. OBJECTIVE: To study the biocompatibility of electrospun poly (lactide-co-glycolide)/polyethylene glycol (PLGA-PEG) nanofibrous scaffolds with mouse neural stem celsin vitro. METHODS:Neural stem cels were isolated from embryos of CD-1 mice at 15 embryonic days. Electrospinning was used to prepare PLGA and PLGA-PEG nanofibrous scaffolds. Scanning electron microscope was used for scanning observation of scaffolds. The 5th passage neural stem cels were seeded onto PLGA and PLGA-PEG scaffolds respectively, and culturedin vitro. RESULTS AND CONCLUSION: Interconnected porous network structure was observed in both two kinds of scaffolds under the scanning electron microscope. Fiber diameters and porosities of PLGA and PLGA-PEG scaffolds showed no significant differences (P > 0.05). Cel Counting Kit-8 detection showed neural stem cels grew wel on both two kinds of scaffolds and the absorbance value of two groups increased continuously with incubation time (1, 3, 5, 7, 9, 11 days). And there were statisticaly significant differences in the absorbance values between two groups at each time point (P < 0.05). Moreover, the cel adhesion rate was significantly higher in the PLGA-PEG group than in the PLGA group at 3, 6, 9 hours of culture (P < 0.05). Hoechst 33342 staining showed normal morphology and quality of the nuclei, and significantly more cels were observed in the PLGA-PEG group than the PLGA group (P < 0.05). Under the scanning electron microscope, compared with the PLGA scaffold, the PLGA-PEG scaffold was better for growth and matrix secretion of neural stem cels. In conclusion, PLGA-PEG nanofibrous scaffolds prepared by electrospinning are safe, non-toxic and suitable for neural stem cels growth with wel biocompatibility, appropriate aperture and porosity.