CAJ | 학술논문

背景：有研究表明，许旺细胞神经移植复合体具有极强修复自体神经缺损作用，并且许旺细胞在神经再生过程中发挥至关重要的作用。目的：比较不同浓度许旺细胞神经移植复合体修复自体神经缺损时周围神经的再生效果。方法：建立坐骨神经缺损模型大鼠。原代培养大鼠许旺细胞，构建聚乳酸-聚羟基乙酸共聚物管-细胞外基质凝胶-许旺细胞神经移植复合体修复坐骨神经缺损模型大鼠。按许旺细胞浓度的不同分为105，106，107，108，109 L-1结果与结论：建模后3，6和12周，含许旺细胞各浓度的神经移植复合体组各时间点神经传导速率均高于对照组(P <0.01)，其中10浓度组，对照组不含许旺细胞。分别于建模后3，6和12周，行运动神经传导速度的测定。建模后12周各组胫骨前肌湿质量测量和组织学观察。8 L-1组运动神经传导速度优于其他各浓度组(P <0.05)。建模后12周，大鼠胫骨前肌苏木精-伊红染色显示，各浓度许旺细胞神经移植复合体组正常肌纤维数均多于对照组(P <0.05)。其中许旺细胞浓度108，109 L-1浓度组胫骨前肌形态恢复较好，肌纤维细条样、波浪状，同向而行，长短、粗细及疏密大致一致。结果证实，108 L-1许旺细胞神经聚乳酸-聚羟基乙酸共聚物移植复合体对缺损坐骨神经再生的促进作用较好。
배경：유연구표명，허왕세포신경이식복합체구유겁강수복자체신경결손작용，병차허왕세포재신경재생과정중발휘지관중요적작용。목적：비교불동농도허왕세포신경이식복합체수복자체신경결손시주위신경적재생효과。방법：건립좌골신경결손모형대서。원대배양대서허왕세포，구건취유산-취간기을산공취물관-세포외기질응효-허왕세포신경이식복합체수복좌골신경결손모형대서。안허왕세포농도적불동분위105，106，107，108，109 L-1결과여결론：건모후3，6화12주，함허왕세포각농도적신경이식복합체조각시간점신경전도속솔균고우대조조(P <0.01)，기중10농도조，대조조불함허왕세포。분별우건모후3，6화12주，행운동신경전도속도적측정。건모후12주각조경골전기습질량측량화조직학관찰。8 L-1조운동신경전도속도우우기타각농도조(P <0.05)。건모후12주，대서경골전기소목정-이홍염색현시，각농도허왕세포신경이식복합체조정상기섬유수균다우대조조(P <0.05)。기중허왕세포농도108，109 L-1농도조경골전기형태회복교호，기섬유세조양、파랑상，동향이행，장단、조세급소밀대치일치。결과증실，108 L-1허왕세포신경취유산-취간기을산공취물이식복합체대결손좌골신경재생적촉진작용교호。
BACKGROUND:Studies have shown that nerve grafts with Schwann cels can repair peripheral nerve defect and Schwann cels have an important role in nerve regeneration. OBJECTIVE: To observe the rehabilitation status of neutral function after sciatic nerve injury in rats bridged by nerve grafts with Schwann cels. METHODS: A rat model of sciatic nerve injury was established, and schwann cels were primarily cultured. Then, the rat model was repaired with polylactic-co-glycolic acid copolymer-extracelular matrix gel-Schwann cels complex. According to different concentrations of Schwann cels, there were five cel groups from 105/L to 109 RESULTS AND CONCLUSION: The nerve conduction velocities in the cel groups were al higher than that in the control group at 3, 6, 12 weeks after modeling (P < 0.01), and it was highest in the 10 and a control group. The nerve conduction velocity was detected respectively at 3, 6, 12 weeks after modeling; the e tibialis anterior muscle gravity was detected and histological observation was done at 12 weeks. 8/L (P < 0.05). At 12 weeks, hematoxylin-eosin staining of the tibialis anterior muscle showed that the number of normal muscle fibers was higher in the cel groups than the control group (P < 0.05). In the 108/L and 109 and had similar length, thickness and density. These findings indicate that polylactic-co-glycolic acid complex with 10/L groups, the morphology of tibialis anterior muscle recovered wel; the muscle fibers were in strip-like and wavy shapes, grew in the same direction, 8/L Schwann cels is better to promote sciatic nerve regeneration.