CAJ | 학술논문

背景：凹凸棒黏土质轻，吸水性强，湿时具有黏性和可塑性等特性，被广泛应用于各个领域，但将凹凸棒黏土作为骨修复材料鲜有报道。目的：将胶原蛋白与不同比例凹凸棒黏土混合，构建一种新型组织工程支架材料，以期运用于骨修复。方法：将胶原蛋白和凹凸棒黏土以干质量比为1∶0、5∶1、4∶1、3∶1的比例混合均匀，冷冻干燥，制备支架材料，扫描电镜观察材料的表观特征，检测1%京尼平交联前后材料的孔隙率和质量吸水率。将4组比例材料分别与大鼠骨髓间充质干细胞共培养，培养3 d及7 d分别进行扫描电镜观察及苏木精-伊红染色。将1%京尼平交联后的4组比例材料分别植入大鼠体内，1个月后观察材料降解情况。结果与结论：材料呈多孔海绵状，交联前材料的孔隙率在60%-80%之间，交联后材料的孔隙率在40%-65%之间，4组之间交联前后孔隙率比较差异无显著意义(P >0.05)。4组材料交联后的质量吸水率较交联前显著降低(P <0.05)。胶原蛋白-凹凸棒黏土支架材料具有良好的细胞相容性，在动物体内可以完全降解并被吸收，但不同比例配比降解速率不一，无炎症反应。
배경：요철봉점토질경，흡수성강，습시구유점성화가소성등특성，피엄범응용우각개영역，단장요철봉점토작위골수복재료선유보도。목적：장효원단백여불동비례요철봉점토혼합，구건일충신형조직공정지가재료，이기운용우골수복。방법：장효원단백화요철봉점토이간질량비위1∶0、5∶1、4∶1、3∶1적비례혼합균균，냉동간조，제비지가재료，소묘전경관찰재료적표관특정，검측1%경니평교련전후재료적공극솔화질량흡수솔。장4조비례재료분별여대서골수간충질간세포공배양，배양3 d급7 d분별진행소묘전경관찰급소목정-이홍염색。장1%경니평교련후적4조비례재료분별식입대서체내，1개월후관찰재료강해정황。결과여결론：재료정다공해면상，교련전재료적공극솔재60%-80%지간，교련후재료적공극솔재40%-65%지간，4조지간교련전후공극솔비교차이무현저의의(P >0.05)。4조재료교련후적질량흡수솔교교련전현저강저(P <0.05)。효원단백-요철봉점토지가재료구유량호적세포상용성，재동물체내가이완전강해병피흡수，단불동비례배비강해속솔불일，무염증반응。
, 1, 2 (1Colege of Life Sciences and Technology, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China;2Institute of Bone Injury, Lanzhou General Hospital of Lanzhou Military Area Command, Lanzhou 730050, Gansu Province, China) <br> BACKGROUND:Attapulgite clay is light with strong water absorption, and it has viscosity and plasticity in wet condition, which has been widely used in various fields. However, there is rarely report about attapulgite clay as bone repair material. OBJECTIVE:To mix colagen with different proportion of attapulgite clay to fabricate a new type of tissue engineering scaffold material for bone repair. METHODS:Colagen and attapulgite clay were melted by dry weight ratio of 1:0, 5:1, 4:1, 3:1 to fabricate scaffold materials. The apparent characteristics of the materials were observed by scanning electron microscopy, the porosity and water absorption of quality of materials were determined before and after 1% genipin cross-linked. Rat bone marrow mesenchymal stem cels were inoculated on the four different materials. The materials were observed by scanning electron microscopy after 3 days. And the materials were stained by hematoxylin-eosin staining after 7 days. The four different materials cross-linked by 1% genipin were implanted into rats and the degradation of materials was observed 1 month after implantation. RESULTS AND CONCLUSION:Materials were sponge and porous. The porosity of materials was between 60% and 80% before cross-linked, while the porosity of materials was between 40% and 65% after cross-linked. There was no significant difference among four groups (P > 0.05). The water absorption of quality of the materials was significantly reduced after cross-linked (P < 0.05). The colagen-attapulgite clay scaffolds have good biocompatibility and can be completely degraded and absorbed in vivo without inflammation. The colagen-attapulgite clay materials of different ratios have different biodegradation rates.