CAJ | 학술논문

背景：激光应用于口腔临床主要用于龋齿的早期诊断、龋坏组织的清除、活髓切断术牙髓止血、牙本质过敏治疗、感染的根管消毒、牙周炎治疗。目的：观察炎症牙髓使用激光处理后碱性成纤维细胞生长因子的变化。方法：5只成年健康beagle犬，每只随机选择6颗牙齿，分为3组，每组10颗，分别使用激光、生理盐水、庆大霉素处理，于术后第1，2，3天用纸尖收集牙髓分泌物检测碱性成纤维细胞生长因子的含量，并进行牙髓苏木精-伊红染色和免疫组织化学检查。结果与结论：生理盐水处理组、庆大霉素处理组及激光处理组第3天碱性成纤维细胞生长因子表达量均明显多于第1天和第2天，第1天和第2天比较差异无显著性意义，3种处理方法之间比较差异无显著性意义。牙髓苏木精-伊红染色和免疫组织学检查显示，髓腔血管状况良好，牙髓排列紧密良好。结果表明，激光治疗对炎症牙髓中碱性成纤维细胞生长因子的释放没有影响。提示激光在口腔临床上的应用是安全、方便、有效的，不会对炎症牙髓组织造成损伤。
배경：격광응용우구강림상주요용우우치적조기진단、우배조직적청제、활수절단술아수지혈、아본질과민치료、감염적근관소독、아주염치료。목적：관찰염증아수사용격광처리후감성성섬유세포생장인자적변화。방법：5지성년건강beagle견，매지수궤선택6과아치，분위3조，매조10과，분별사용격광、생리염수、경대매소처리，우술후제1，2，3천용지첨수집아수분비물검측감성성섬유세포생장인자적함량，병진행아수소목정-이홍염색화면역조직화학검사。결과여결론：생리염수처리조、경대매소처리조급격광처리조제3천감성성섬유세포생장인자표체량균명현다우제1천화제2천，제1천화제2천비교차이무현저성의의，3충처리방법지간비교차이무현저성의의。아수소목정-이홍염색화면역조직학검사현시，수강혈관상황량호，아수배렬긴밀량호。결과표명，격광치료대염증아수중감성성섬유세포생장인자적석방몰유영향。제시격광재구강림상상적응용시안전、방편、유효적，불회대염증아수조직조성손상。
BACKGROUND:The laser applied in the clinical oral medicine is mainly for early diagnosis of caries, removal of carious tissue, pulpotomy for pulp bleeding, dentin hypersensitivity treatment, disinfection of infected root canals, and periodontitis treatment. OBJECTIVE:To explore the changes of basic fibroblast growth factor in inflammatory pulp after laser treatment. METHODS:Five healthy adult beagle dogs were enroled, and six teeth were randomly selected from each dog. Then, these teeth were randomly divided into three groups, 10 teeth in a group, and they were treated with laser irradiation, saline and gentamicin irrigation respectively. At days 1, 2, 3 after operation, exudates from the pulp were colected for detection of basic fibroblast growth factor expression. Meanwhile, the dental pulp was detected by hematoxylin-eosin staining and immunohistochemistry method. RESULTS AND CONCLUSION:In the al three groups, the expression of basic fibroblast growth factor at day 3 was significantly higher than that at days 1 and 2, and there were no differences between days 1 and 2. Moreover, these three groups also showed no significant differences. Under hematoxylin-eosin and immunohistochemical staining, blood vessels in the pulp cavity were in good condition and the pulp arranged tightly. These findings suggest that the laser is safe, convenient and effective for oral clinical application, and has no injury to the inflammatory pulp.