CAJ | 학술논문

目的：探讨长链非编码RNA HOX 转录反义RNA( HOTAIR)在非小细胞肺癌( NSCLC)中的表达,及与临床病理及预后的关系。方法收集我院60例NSCLC手术切除标本(包括癌组织及对应的正常肺组织),qRT-PCR检测60例NSCLC中HOTAIR的表达,并分析其与临床病理及预后的关系。结果肺癌组织中的HOTAIR的表达水平(5．18±2．44)显著高于正常肺组织中的表达水平(3．54±1．65)( P <0．001)。NSCLC患者肿瘤组织中HOTAIR的表达水平与肿瘤的大小、分化程度及临床分期明显相关( P<0．05)。 HO-TAIR高表达者生存时间明显低于低表达者,差异有显著统计学意义(P<0．05)。 HOTAIR高表达组和低表达组的中位生存时间分别为46．03和52.9个月。结论 HOTAIR 在 NSCLC 组织中显著高表达,可能成为NSCLC判断预后和治疗的新的分子标记物。
목적：탐토장련비편마RNA HOX 전록반의RNA( HOTAIR)재비소세포폐암( NSCLC)중적표체,급여림상병리급예후적관계。방법수집아원60례NSCLC수술절제표본(포괄암조직급대응적정상폐조직),qRT-PCR검측60례NSCLC중HOTAIR적표체,병분석기여림상병리급예후적관계。결과폐암조직중적HOTAIR적표체수평(5．18±2．44)현저고우정상폐조직중적표체수평(3．54±1．65)( P <0．001)。NSCLC환자종류조직중HOTAIR적표체수평여종류적대소、분화정도급림상분기명현상관( P<0．05)。 HO-TAIR고표체자생존시간명현저우저표체자,차이유현저통계학의의(P<0．05)。 HOTAIR고표체조화저표체조적중위생존시간분별위46．03화52.9개월。결론 HOTAIR 재 NSCLC 조직중현저고표체,가능성위NSCLC판단예후화치료적신적분자표기물。
Objective To examine the expression pattern of HOTAIR in NSCLC and to evaluate its clinical significance in tumor progression. Methods 60 specimens of NSCLC tissues and the paired normal tissues after sur-gery were collected from June 2007 to February 2008. Quantitative real-time polymerase chain reaction for HOTAIR expression was performed on cancerous and normal lung tissues. Results The expression level of HOTAIR was up-regulated in tumor tissues as compared with the paired normal lung tissues ( 5. 18 ± 2. 44 vs 3. 54 ± 1. 65 ) ( P <0. 05). The expression of HOTAIR was associated with differentiation, tumor size and TNM stages (P<0. 05). The high expression of HOTAIR was significantly correlated with overall survival of NSCLC (P<0. 05). The median sur-vival time of HOTAIR between the high expression group and the low expression group were 46. 03 and 52. 9 months, respectively. Conclusion The expression of HOTAIR is upregulated in tumor tissues and can serve as a potential prognostic and therapeutic biomarker for NSCLC.