临床肺科杂志
臨床肺科雜誌
림상폐과잡지
JOUNAL OF CLINICAL PULMONARY MEDICINE
2015年
1期
83-86
,共4页
慢性阻塞性肺病%Th17细胞%调节T细胞%炎症因子
慢性阻塞性肺病%Th17細胞%調節T細胞%炎癥因子
만성조새성폐병%Th17세포%조절T세포%염증인자
COPD%Th17 cells%regulatory T cells%inflammatory cytokines
目的：探讨不同分期老年慢性阻塞性肺病( COPD)患者外周血Th17、调节T细胞( Treg)细胞及相关炎症因子水平的变化及其与患者肺功能的关系。方法选取2012年1月至2014年1月在本院呼吸内科就诊的68例COPD患者(其中稳定期32例,急性期36例)为研究对象,另选取30例健康体检者为对照组。应用流式细胞仪测定两组外周血CD4+ Th17、CD4+ CD2+5 Treg细胞的比率。应用ELISA法测定两组白细胞介素-6,10,17,21,22(IL-6,10,17,21,22)及转化生长因子β(TGF-β)水平。结果急性期组患者FEV1、FEV1/FVC水平显著低于稳定期及对照组(P<0．05),而稳定期组低于对照组(P<0．05)。急性期组CD4+ Th17细胞比率高于稳定期及对照组, CD4+ CD2+5 Treg细胞的比率则低于稳定期及对照组,差异有统计学意义( P<0．05)。急性期组IL-6,17,21,22水平高于稳定期及对照组,而IL-10、TGF-β水平低于稳定期及对照组,差异有统计学意义(P<0．05)。 FEV1与IL-6,17,21,22呈负相关,而与IL-10、TGF-β水平呈正相关, FEV1/FVC与IL-6,17,21,22呈负相关,而与IL-10、TGF-β水平呈正相关。结论 Th17/Treg比例失衡可导致机体促炎因子生成增多,抑制炎症作用不足,促进气道炎症反应,导致患者呼吸功能障碍,加重COPD病情。
目的：探討不同分期老年慢性阻塞性肺病( COPD)患者外週血Th17、調節T細胞( Treg)細胞及相關炎癥因子水平的變化及其與患者肺功能的關繫。方法選取2012年1月至2014年1月在本院呼吸內科就診的68例COPD患者(其中穩定期32例,急性期36例)為研究對象,另選取30例健康體檢者為對照組。應用流式細胞儀測定兩組外週血CD4+ Th17、CD4+ CD2+5 Treg細胞的比率。應用ELISA法測定兩組白細胞介素-6,10,17,21,22(IL-6,10,17,21,22)及轉化生長因子β(TGF-β)水平。結果急性期組患者FEV1、FEV1/FVC水平顯著低于穩定期及對照組(P<0．05),而穩定期組低于對照組(P<0．05)。急性期組CD4+ Th17細胞比率高于穩定期及對照組, CD4+ CD2+5 Treg細胞的比率則低于穩定期及對照組,差異有統計學意義( P<0．05)。急性期組IL-6,17,21,22水平高于穩定期及對照組,而IL-10、TGF-β水平低于穩定期及對照組,差異有統計學意義(P<0．05)。 FEV1與IL-6,17,21,22呈負相關,而與IL-10、TGF-β水平呈正相關, FEV1/FVC與IL-6,17,21,22呈負相關,而與IL-10、TGF-β水平呈正相關。結論 Th17/Treg比例失衡可導緻機體促炎因子生成增多,抑製炎癥作用不足,促進氣道炎癥反應,導緻患者呼吸功能障礙,加重COPD病情。
목적：탐토불동분기노년만성조새성폐병( COPD)환자외주혈Th17、조절T세포( Treg)세포급상관염증인자수평적변화급기여환자폐공능적관계。방법선취2012년1월지2014년1월재본원호흡내과취진적68례COPD환자(기중은정기32례,급성기36례)위연구대상,령선취30례건강체검자위대조조。응용류식세포의측정량조외주혈CD4+ Th17、CD4+ CD2+5 Treg세포적비솔。응용ELISA법측정량조백세포개소-6,10,17,21,22(IL-6,10,17,21,22)급전화생장인자β(TGF-β)수평。결과급성기조환자FEV1、FEV1/FVC수평현저저우은정기급대조조(P<0．05),이은정기조저우대조조(P<0．05)。급성기조CD4+ Th17세포비솔고우은정기급대조조, CD4+ CD2+5 Treg세포적비솔칙저우은정기급대조조,차이유통계학의의( P<0．05)。급성기조IL-6,17,21,22수평고우은정기급대조조,이IL-10、TGF-β수평저우은정기급대조조,차이유통계학의의(P<0．05)。 FEV1여IL-6,17,21,22정부상관,이여IL-10、TGF-β수평정정상관, FEV1/FVC여IL-6,17,21,22정부상관,이여IL-10、TGF-β수평정정상관。결론 Th17/Treg비례실형가도치궤체촉염인자생성증다,억제염증작용불족,촉진기도염증반응,도치환자호흡공능장애,가중COPD병정。
Objective To investigate the inflammatory factors and Th17 and Treg cell ratio of different stages of elderly patients with COPD. Methods The study selected 68 COPD patients as the observation group, including 32 stable COPD patients and 36 AECOPD patients, and 30 healthy people as the control group. The ratio of CD4+Th17 and CD4+CD2+5 Treg cells was detected by flow cytometry, and the levels of IL-6, IL-10, IL-17, IL-21, IL-22 and TGF-β were detected by ELISA. Results The levels of FEV1 and FEV1/FVC were the lowest in the AECO-PD group, followed by the stable COPD group and the control group ( P<0. 05 ) . The ratio of CD4+Th17 cells was higher and the ratio of CD4+CD2+5 Treg cells was lower in the AECOPD group than in the stable group and the control group (P<0. 05). The levels of IL-6, IL-17, IL-21 and IL-22 were higher and the levels of IL-10 and TGF-βwere lower in the AECOPD group than in the stable group and the control group (P<0. 05). The level of FEV1 was nega-tively correlated with IL-6, IL-17, IL-21 and IL-22, and it was positively correlated with IL-10 and TGF-β. The val-ue of FEV1/FVC was negatively correlated with IL-6, IL-17, IL-21 and IL-22, and it was negatively correlated with IL-10 and TGF-β. Conclusion The imbalance of Th17/Treg can cause the body to generate an increase in pro-in-flammatory cytokines, leading to respiratory dysfunction in COPD patients and making their situation worsen.
