临床肺科杂志
臨床肺科雜誌
림상폐과잡지
JOUNAL OF CLINICAL PULMONARY MEDICINE
2015年
1期
30-33
,共4页
马晓辉%张翠云%王继东%李养军
馬曉輝%張翠雲%王繼東%李養軍
마효휘%장취운%왕계동%리양군
乌司他丁%脓毒症%肺组织%TNF-α%IL-1β%Bcl-2
烏司他丁%膿毒癥%肺組織%TNF-α%IL-1β%Bcl-2
오사타정%농독증%폐조직%TNF-α%IL-1β%Bcl-2
ulinastatin%sepsis%lung tissue%TNF-α%IL-1β%Bcl-2
目的：观察不同剂量乌司他丁对脓毒症大鼠炎症相关因子的影响。方法65只雌性SD大鼠随机分为5组：正常组(空白对照)、模型组、乌司他丁U1(10万u/kg)组、乌司他丁U2(30万u/kg)组、乌司他丁U3(60万u/kg)组。脓毒症大鼠造模成功后,按不同剂量经腹腔给药,分别于用药后3、6、12 h给予大鼠取血,ELISA法检测各组大鼠血清TNF-α、IL-1β水平,免疫组化检测肺组织Bcl-2蛋白的表达情况。结果模型组及乌司他丁组各时间点的血清TNF-α、IL-1β均高于正常组( P<0．05)；乌司他丁组各时间点血清TNF-α、IL-1β的表达低于模型组(P<0．05)；而其中U2组的TNF-α、IL-1β的血清浓度与U1组及U3组的差别有统计学意义(P<0．05)。肺组织切片HE染色可见U2组的炎症反应较模型组明显减轻；免疫组化提示U2组的Bcl-2蛋白表达强于模型组。结论不同剂量乌司他丁均可减轻脓毒症大鼠的炎症反应,且在剂量30万u/kg时疗效最显著。
目的：觀察不同劑量烏司他丁對膿毒癥大鼠炎癥相關因子的影響。方法65隻雌性SD大鼠隨機分為5組：正常組(空白對照)、模型組、烏司他丁U1(10萬u/kg)組、烏司他丁U2(30萬u/kg)組、烏司他丁U3(60萬u/kg)組。膿毒癥大鼠造模成功後,按不同劑量經腹腔給藥,分彆于用藥後3、6、12 h給予大鼠取血,ELISA法檢測各組大鼠血清TNF-α、IL-1β水平,免疫組化檢測肺組織Bcl-2蛋白的錶達情況。結果模型組及烏司他丁組各時間點的血清TNF-α、IL-1β均高于正常組( P<0．05)；烏司他丁組各時間點血清TNF-α、IL-1β的錶達低于模型組(P<0．05)；而其中U2組的TNF-α、IL-1β的血清濃度與U1組及U3組的差彆有統計學意義(P<0．05)。肺組織切片HE染色可見U2組的炎癥反應較模型組明顯減輕；免疫組化提示U2組的Bcl-2蛋白錶達彊于模型組。結論不同劑量烏司他丁均可減輕膿毒癥大鼠的炎癥反應,且在劑量30萬u/kg時療效最顯著。
목적：관찰불동제량오사타정대농독증대서염증상관인자적영향。방법65지자성SD대서수궤분위5조：정상조(공백대조)、모형조、오사타정U1(10만u/kg)조、오사타정U2(30만u/kg)조、오사타정U3(60만u/kg)조。농독증대서조모성공후,안불동제량경복강급약,분별우용약후3、6、12 h급여대서취혈,ELISA법검측각조대서혈청TNF-α、IL-1β수평,면역조화검측폐조직Bcl-2단백적표체정황。결과모형조급오사타정조각시간점적혈청TNF-α、IL-1β균고우정상조( P<0．05)；오사타정조각시간점혈청TNF-α、IL-1β적표체저우모형조(P<0．05)；이기중U2조적TNF-α、IL-1β적혈청농도여U1조급U3조적차별유통계학의의(P<0．05)。폐조직절편HE염색가견U2조적염증반응교모형조명현감경；면역조화제시U2조적Bcl-2단백표체강우모형조。결론불동제량오사타정균가감경농독증대서적염증반응,차재제량30만u/kg시료효최현저。
Objective To research the effect of different doses of ulinastatin on the levels of inflammation-related cytokines in rats suffering sepsis. Methods 65 female SD rats were randomly divided into five groups: the normal group (blank control), the model group, the ulinastatin U1 group (100,000u/kg), the ulinastatin U2 group (300,000 u/kg), and the ulinastatin U3 group (600,000 u/kg). The rat models of sepsis were allocated to intraper-itoneal injection according to different dosing when the sepsis symptoms appeared. Their blood was extracted 3, 6 and 12 hours after the treatment. The levels of TNF-αand IL-1βin serum were detected by enzyme-linked immunosorbent assay ( ELISA) , and the expression of Bcl-2 protein in lung tissues was detected by immunohistochemical technique. Results At all the time points, the serum levels of TNF-α and IL-1β were higher in the ulinastatin group and the model group than in the control group ( P<0. 05 ) , and they were lower in the ulinastatin group than in the model group (P<0. 05). The levels of TNF-αand IL-1βin the U2 group had statistically significant difference with the U1 group and the U3 group ( P<0. 05 ) . Lung biopsy in HE staining showed that the inflammatory response in the U2 group was significantly lighter than the non-drug model group. At the same time, the expression of Bcl-2 protein in lung tissue of the U2 group was stronger than those of the model group. Conclusion Different doses of ulinastatin may reduce the inflammatory response in septic rats, and the dosage of 300,000 u/kg has the most significant effect on septic rats.
