热带病与寄生虫学
熱帶病與寄生蟲學
열대병여기생충학
TROPICAL DISEASES AND PARASITOLOGY
2014年
4期
187-189,193
,共4页
常宏伟%李开春%马泰%杨卫%张凤%张丽梅%孙林%李娟%李伟
常宏偉%李開春%馬泰%楊衛%張鳳%張麗梅%孫林%李娟%李偉
상굉위%리개춘%마태%양위%장봉%장려매%손림%리연%리위
结核分枝杆菌%耐药%katG基因%rpoB基因%突变
結覈分枝桿菌%耐藥%katG基因%rpoB基因%突變
결핵분지간균%내약%katG기인%rpoB기인%돌변
Mycobacterium tuberculosis%Drug resistance%katG%rpoB%Mutation
目的：分析临床分离的结核分枝杆菌katG和rpoB基因的突变情况与耐药之间的关系，了解六安地区耐药结核分枝杆菌的基因突变特征。方法采用比例法对六安地区65株结核分枝杆菌临床分离株进行药敏试验；通过特异性引物，对目的基因片段katG和rpoB进行扩增、测序后进行分析。结果60株结核分枝杆菌中分别有有9株耐异烟肼和14株耐利福平，其中同时耐异烟肼和利福平的6株。9株耐异烟肼菌株中，4株katG在315（A GC→ACC或ACA）位点发生突变，占44.4％；14株耐利福平菌株中，11株rpoB分别在516（GAC→GTC）、526(CAC→CGC或TAC)和531(TCG→TTG)位点发生突变，占总耐药菌株的78.6％；6株同时对利福平和异烟肼耐药，其中5株katG或rpoB基因发生突变，占83.3％。结论六安地区结核分枝杆菌耐异烟肼和耐利福平分别主要是由katG和rpoB基因突变引起，其中耐异烟肼主要在315（AGC→ACC或ACA）位，而耐利福平在516（GAC→GTC）、526(CAC→CGC或TAC)和531(TCG→TTG)位，都发生了突变。
目的：分析臨床分離的結覈分枝桿菌katG和rpoB基因的突變情況與耐藥之間的關繫，瞭解六安地區耐藥結覈分枝桿菌的基因突變特徵。方法採用比例法對六安地區65株結覈分枝桿菌臨床分離株進行藥敏試驗；通過特異性引物，對目的基因片段katG和rpoB進行擴增、測序後進行分析。結果60株結覈分枝桿菌中分彆有有9株耐異煙肼和14株耐利福平，其中同時耐異煙肼和利福平的6株。9株耐異煙肼菌株中，4株katG在315（A GC→ACC或ACA）位點髮生突變，佔44.4％；14株耐利福平菌株中，11株rpoB分彆在516（GAC→GTC）、526(CAC→CGC或TAC)和531(TCG→TTG)位點髮生突變，佔總耐藥菌株的78.6％；6株同時對利福平和異煙肼耐藥，其中5株katG或rpoB基因髮生突變，佔83.3％。結論六安地區結覈分枝桿菌耐異煙肼和耐利福平分彆主要是由katG和rpoB基因突變引起，其中耐異煙肼主要在315（AGC→ACC或ACA）位，而耐利福平在516（GAC→GTC）、526(CAC→CGC或TAC)和531(TCG→TTG)位，都髮生瞭突變。
목적：분석림상분리적결핵분지간균katG화rpoB기인적돌변정황여내약지간적관계，료해륙안지구내약결핵분지간균적기인돌변특정。방법채용비례법대륙안지구65주결핵분지간균림상분리주진행약민시험；통과특이성인물，대목적기인편단katG화rpoB진행확증、측서후진행분석。결과60주결핵분지간균중분별유유9주내이연정화14주내리복평，기중동시내이연정화리복평적6주。9주내이연정균주중，4주katG재315（A GC→ACC혹ACA）위점발생돌변，점44.4％；14주내리복평균주중，11주rpoB분별재516（GAC→GTC）、526(CAC→CGC혹TAC)화531(TCG→TTG)위점발생돌변，점총내약균주적78.6％；6주동시대리복평화이연정내약，기중5주katG혹rpoB기인발생돌변，점83.3％。결론륙안지구결핵분지간균내이연정화내리복평분별주요시유katG화rpoB기인돌변인기，기중내이연정주요재315（AGC→ACC혹ACA）위，이내리복평재516（GAC→GTC）、526(CAC→CGC혹TAC)화531(TCG→TTG)위，도발생료돌변。
Objective To investigate the characteristics of mutations in the rpoB and katG genes(clini?cal isolates) leading to drug-resistance in Mycobacterium tuberculosis(M. tuberculosis) in Lu’an area. Methods Drug sensitive test was performed in 65 strains of M. tuberculosis clinically isolated from the sputum samples col?lected in Lu'an area, and the gene fragments of rpoB and katG were amplified with specific primers, sequenced and analyzed. Results Of the 60 strains of M. tuberculosis, 9 were mutations involved in resistance against isoni?azid, 14 against rifampin, and 6 against both. The mutation for the 4 in 9 isoniazid-resistant strains(katG gene) occurred at codon 315(codon change from AGG to ACC or ACA, 44.4%), and 11 of 14 rifampin resistant strains(rpoB gene) occurred at codon 516(GAC→GTC), 526(CAC→CGC or TAC) and 531(TCG→TTG, 78.6 %). Five in 6 strains resistant to both isoniazid and rifampin had mutations, which accounted for 83.3%. Conclusion Drug-resistance M. tuberculosis in Lu’an area is associated with katG and rpoB gene mutation, in which the katG gene primarily occurs at codon 315(AGC→ACC or ACA), and the rpoB at 516(GAC→GTC), 526(CAC→CGC or TAC) and 531(TCG→TTG).
