广西林业科学
廣西林業科學
엄서임업과학
GUANGXI FORESTRY SCIENCE
2014年
4期
405-408
,共4页
招礼军%龙永宁%朱栗琼%刘威%莫世宇%罗叶红
招禮軍%龍永寧%硃慄瓊%劉威%莫世宇%囉葉紅
초례군%룡영저%주률경%류위%막세우%라협홍
灰木莲%花粉%离体萌发%生活力%花粉管
灰木蓮%花粉%離體萌髮%生活力%花粉管
회목련%화분%리체맹발%생활력%화분관
Manglietia glauca%pollen%in vitro germination%viability%pollen tube
以灰木莲( Manglietia glauca)的新鲜花粉为材料,研究不同浓度蔗糖、硼酸和氯化钙组合的培养基对灰木莲花粉离体萌发的影响,并用TTC染色法、 I-IK染色法和无机酸法对灰木莲的花粉生活力进行了测定。结果表明:在pH值6.0时,以150 g/L蔗糖+50 mg/L硼酸+100 mg/L氯化钙+7 g/L琼脂的培养基配方最适合灰木莲花粉的培养,离体萌发率达到62.2%,花粉管生长良好;无机酸法不适宜灰木莲花粉生活力的检测;TTC染色法和I-IK染色法的测定结果与离体萌发法的结果较接近,分别为60.6%和64.1%,但I-IK染色法在观察判断上较为困难。因此, TTC染色法是快速检测灰木莲花粉生活力的最佳方法。
以灰木蓮( Manglietia glauca)的新鮮花粉為材料,研究不同濃度蔗糖、硼痠和氯化鈣組閤的培養基對灰木蓮花粉離體萌髮的影響,併用TTC染色法、 I-IK染色法和無機痠法對灰木蓮的花粉生活力進行瞭測定。結果錶明:在pH值6.0時,以150 g/L蔗糖+50 mg/L硼痠+100 mg/L氯化鈣+7 g/L瓊脂的培養基配方最適閤灰木蓮花粉的培養,離體萌髮率達到62.2%,花粉管生長良好;無機痠法不適宜灰木蓮花粉生活力的檢測;TTC染色法和I-IK染色法的測定結果與離體萌髮法的結果較接近,分彆為60.6%和64.1%,但I-IK染色法在觀察判斷上較為睏難。因此, TTC染色法是快速檢測灰木蓮花粉生活力的最佳方法。
이회목련( Manglietia glauca)적신선화분위재료,연구불동농도자당、붕산화록화개조합적배양기대회목연화분리체맹발적영향,병용TTC염색법、 I-IK염색법화무궤산법대회목련적화분생활력진행료측정。결과표명:재pH치6.0시,이150 g/L자당+50 mg/L붕산+100 mg/L록화개+7 g/L경지적배양기배방최괄합회목연화분적배양,리체맹발솔체도62.2%,화분관생장량호;무궤산법불괄의회목연화분생활력적검측;TTC염색법화I-IK염색법적측정결과여리체맹발법적결과교접근,분별위60.6%화64.1%,단I-IK염색법재관찰판단상교위곤난。인차, TTC염색법시쾌속검측회목연화분생활력적최가방법。
The fresh pollen of Manglietia glauca was used as material to study the effect of mediums with different concentrations of sucrose, boric acid and calcium chloride on in vitro pollen germination . And the pollen viabilities of M. glauca were tested by using 2 , 3 , 5-triphenyltetrazolium chloride staining ( TTC) , iodine-potassium iodide staining ( I-IK) and mineral acid test. The results showed that when the pH was 6. 0, the optimum medium consisting of 150 g/L sucrose, 50 mg/L boric acid, 100 mg/L calci-um chloride and 7 g/L agar was suitable for M. glauca pollen cultured. The in vitro germination rate was 62. 2% and the pollen tube grew well. The mineral acid test was unsuitable for detecting the pollen via-bility. The results tested by TTC and I-IK methods were similar to that of in vitro germination method with pollen viabilities 60. 6% and 64. 1% respectively. However, it was difficult to distinguish the stained pollen grains using the I-IK method. The TTC method was the optimum test method for testing pollen via-bility of M. glauca rapidly.