CAJ | 학술논문

以华山松( Pinus armandi)针叶为实验材料,分别采取经典CTAB法、简易CTAB法、改良CTAB法和Zigenhagen法提取华山松基因组DNA,并通过琼脂糖凝胶电泳、紫外分光光度计分析、标准差和标准误、卡方检验和SPSS软件分析。通过对实验数据进行卡方检验,所有数据的差异是由DNA提取方法所引起的,不是偶然误差造成的。对( OD260- OD320)/( OD280- OD320)的标准差和标准误分析表明：改良CTAB法的标准差和标准误最小,经典CTAB法次之,再其次是简易CTAB法,说明改良CTAB法稳定性最好,数据精确度最好。通过紫外分光光度计和SPSS分析,经典CTAB法存在RNA污染,( OD260- OD320)/( OD280- OD320)的比值大于1．90,简易CTAB法和Zigenhagen法存在少量蛋白质污染,( OD260- OD320)/( OD280- OD320)的比值小于1．80,而改良CTAB法(OD260- OD320)/(OD280- OD320)的比值是1．855,接近理论值(1．80),且标准差和标准误最小,数据稳定性和精确性最好,提取DNA纯度最好。
이화산송( Pinus armandi)침협위실험재료,분별채취경전CTAB법、간역CTAB법、개량CTAB법화Zigenhagen법제취화산송기인조DNA,병통과경지당응효전영、자외분광광도계분석、표준차화표준오、잡방검험화SPSS연건분석。통과대실험수거진행잡방검험,소유수거적차이시유DNA제취방법소인기적,불시우연오차조성적。대( OD260- OD320)/( OD280- OD320)적표준차화표준오분석표명：개량CTAB법적표준차화표준오최소,경전CTAB법차지,재기차시간역CTAB법,설명개량CTAB법은정성최호,수거정학도최호。통과자외분광광도계화SPSS분석,경전CTAB법존재RNA오염,( OD260- OD320)/( OD280- OD320)적비치대우1．90,간역CTAB법화Zigenhagen법존재소량단백질오염,( OD260- OD320)/( OD280- OD320)적비치소우1．80,이개량CTAB법(OD260- OD320)/(OD280- OD320)적비치시1．855,접근이론치(1．80),차표준차화표준오최소,수거은정성화정학성최호,제취DNA순도최호。
Using leaves of Pinus armandi as experimental materials, genomic DNA was extracted using four methods, which were the classical CTAB method, simple CTAB method, modified CTAB method and Zigenhagen method and were analyzed by agarose gel electrophoresis, ultraviolet spectrophotometer, standard deviation and standard error of arithmetic mean, Chi-square test and SPSS. The result of the Chi-square test showed that the differences of the data were derived from the method, not the accidental error. According to the analysis of ( OD260 - OD320 ) / ( OD280 - OD320 ) , the standard deviation and standard error of modified CTAB method was the lowest. The classical CTAB method was the second. The simple CTAB method was the third. It implied that stability and the data precision were the best by modi-fied CTAB method. The results of the ultraviolet spectrophotometer and SPSS showed that the ratio (OD260 - OD320 ) / ( OD280 -OD320 ) of classical CTAB method was greater than 1. 9, which might have RNA pollution. The ratio ( OD260 -OD320 ) / ( OD280 -OD320 ) of simple CTAB method and Zigen-hagen method were less than 1. 80 , which might have little protein pollution. The ratio ( OD260 -OD320 ) / ( OD280 -OD320 ) of modified CTAB method was 1. 855 closed to the theory value (1. 80) with the lowest standard deviation and standard error, which indicated that stability and precision of data were the best and the DNA purity was the best.