CAJ | 학술논문

试验选用硫酸庆大霉素构建小鼠肾病理模型。48只健康小鼠随机分为3组，即健康对照组、试验Ⅰ组和试验Ⅱ组。其中健康对照组不做任何处理，试验Ⅰ组和试验Ⅱ组依次每天腹腔注射硫酸庆大霉素0.075和0.150 mL/只，连续注射7 d，测定小鼠血清中尿素氮、肌酐、蛋白质的含量及肾指数。结果表明，试验Ⅰ组与健康对照组相比，各项指标均差异不显著（P＞0.05）；而试验Ⅱ组与健康对照组相比，各项指标均差异显著（P＜0.05）。结果提示，连续7 d给小鼠腹腔注射0.150 mL/只的硫酸庆大霉素可以成功构建肾病理模型。
시험선용류산경대매소구건소서신병리모형。48지건강소서수궤분위3조，즉건강대조조、시험Ⅰ조화시험Ⅱ조。기중건강대조조불주임하처리，시험Ⅰ조화시험Ⅱ조의차매천복강주사류산경대매소0.075화0.150 mL/지，련속주사7 d，측정소서혈청중뇨소담、기항、단백질적함량급신지수。결과표명，시험Ⅰ조여건강대조조상비，각항지표균차이불현저（P＞0.05）；이시험Ⅱ조여건강대조조상비，각항지표균차이현저（P＜0.05）。결과제시，련속7 d급소서복강주사0.150 mL/지적류산경대매소가이성공구건신병리모형。
The kidney pathological models injured by gentamycin were established in mice. All of the mice were randomly divided into 3 groups:control group, testⅠgroup and test Ⅱ group. The control group had no treatment and the other groups were given gentamycin by intraperitoneal injection respectively with doses of 0.075 and 0.150 mL for 7 days. The levels of urea, creatinine, serum protein and kidney index in mice were detected. All of test items in mice had no signifcant differences between the control group and the testⅠgroup(P>0.05), but had signifcant differences between the control group and the testⅡ group (P<0.05). The results indicate that injecting 0.150 mL gentamycin to mice for 7 days can make ideal pathological model of kidney.