CAJ | 학술논문

利用菌落PCR方法对临床致病菌进行快速鉴定，以天津地区某猪场病死猪胃、肠内容物作为病料进行细菌分离培养，并对所分离的细菌进行药敏试验鉴定。设计16S rRNA通用引物并利用菌落PCR方法扩增所分离细菌的16S rRNA序列，对该序列进行测定和BLAST分析。结果发现，该细菌为一株多重耐药性大肠杆菌。试验建立了仔猪大肠杆菌病的临床快速检测方法。
이용균락PCR방법대림상치병균진행쾌속감정，이천진지구모저장병사저위、장내용물작위병료진행세균분리배양，병대소분리적세균진행약민시험감정。설계16S rRNA통용인물병이용균락PCR방법확증소분리세균적16S rRNA서렬，대해서렬진행측정화BLAST분석。결과발현，해세균위일주다중내약성대장간균。시험건립료자저대장간균병적림상쾌속검측방법。
In order to investigate the application value of polymerase chain reaction (PCR) technique in detection of pathogenic bacteria in piglets, pigs’ stomach, intestinal contents as disease materials from a pig farm in Tianjin area were expected to isolate and cultivate the pathogenic bacteria and test the drug sensitivity. Universal primes were designed to detect 16S rRNA sequences from isolated bacteria by using PCR method and the results were analyzed by the biological software BLAST. The results show that the pathogenic bacteria was a multiple drug resistant type Escherichia coli. A rapid clinical method of detecting colibacillosis of piglet was found in this study.