分析测试技术与仪器
分析測試技術與儀器
분석측시기술여의기
ANALYSIS AND TESTING TECHNOLOGY AND INSTRUMENTS
2014年
4期
197-203
,共7页
毛细管电泳%非衍生化氨基酸%间接紫外检测%太子参
毛細管電泳%非衍生化氨基痠%間接紫外檢測%太子參
모세관전영%비연생화안기산%간접자외검측%태자삼
capillary zone electrophoresis%non-derivatized amino acids%indirect UV detection%Radix Pseudostell
建立了一种简单、快速测定赖氨酸( Lys)、脯氨酸( Pro)、亮氨酸( Leu)、丙氨酸( Ala)、组氨酸( His)、苏氨酸( Thr)、蛋氨酸( Met)、丝氨酸( Ser)和甘氨酸( Gly)的毛细管电泳-间接紫外检测方法.通过研究缓冲液的种类和浓度、缓冲液的pH等分离条件对被测组分分离度和灵敏度的影响,从而优化了分析条件.实验结果表明,用0.1 mol/L氢氧化钠溶液调节14 mmol/L的对氨基苯磺酸至pH为11.2做为运行缓冲液,当分离电压为20 kV时,9组分在12 min内实现了完全分离.实验结果表明,方法能成功用于不同产地太子参药材中9种氨基酸的含量测定.方法重现性良好,迁移时间和峰高的RSD分别小于2.6%和4.5%(n=7).
建立瞭一種簡單、快速測定賴氨痠( Lys)、脯氨痠( Pro)、亮氨痠( Leu)、丙氨痠( Ala)、組氨痠( His)、囌氨痠( Thr)、蛋氨痠( Met)、絲氨痠( Ser)和甘氨痠( Gly)的毛細管電泳-間接紫外檢測方法.通過研究緩遲液的種類和濃度、緩遲液的pH等分離條件對被測組分分離度和靈敏度的影響,從而優化瞭分析條件.實驗結果錶明,用0.1 mol/L氫氧化鈉溶液調節14 mmol/L的對氨基苯磺痠至pH為11.2做為運行緩遲液,噹分離電壓為20 kV時,9組分在12 min內實現瞭完全分離.實驗結果錶明,方法能成功用于不同產地太子參藥材中9種氨基痠的含量測定.方法重現性良好,遷移時間和峰高的RSD分彆小于2.6%和4.5%(n=7).
건립료일충간단、쾌속측정뢰안산( Lys)、포안산( Pro)、량안산( Leu)、병안산( Ala)、조안산( His)、소안산( Thr)、단안산( Met)、사안산( Ser)화감안산( Gly)적모세관전영-간접자외검측방법.통과연구완충액적충류화농도、완충액적pH등분리조건대피측조분분리도화령민도적영향,종이우화료분석조건.실험결과표명,용0.1 mol/L경양화납용액조절14 mmol/L적대안기분광산지pH위11.2주위운행완충액,당분리전압위20 kV시,9조분재12 min내실현료완전분리.실험결과표명,방법능성공용우불동산지태자삼약재중9충안기산적함량측정.방법중현성량호,천이시간화봉고적RSD분별소우2.6%화4.5%(n=7).
An analytical system for lysine(Lys), proline(Pro), leucine(Leu), alanine (Ala), histidine(His), threonine( Thr ) , methionine ( Met ) , serine ( Ser ) and glycine ( Gly ) is established using the capillary zone electrophoresis indirect UV detection method. The experimental conditions including type, concentration and pH of buffer solution were studied in detail. The optimal experimental conditions were as follows:0. 1 mol/L NaOH-14 mmol/L sulfanilic acid solution (pH=11. 2) as the buffer solution, 20 kV as the separation voltage. The nine amino acids could be completely separated in 12 min. Furthermore, the content of the nine amino acids in Radix Pseudostell from different sources were successfully determined. The method showed good reproducibility, RSDs of migration time and peak height were less than 2. 6% and 4. 5% (n = 7), respectively.
