CAJ | 학술논문

目的：探讨ERCC1在非小细胞肺癌中的表达及其临床意义。方法利用免疫组化法检测ERCC1蛋白在160例非小细胞肺癌组织中表达情况，分析ERCC1蛋白表达与非小细胞肺癌临床病理参数的关系及预后的相关性。同时留取这160例术中标本进行原代培养，检测其对DDP的药物敏感性及与ERCC1表达的相关性。结果 ERCC1蛋白主要在细胞核中呈现棕黄色颗粒。肺癌组织ERCC1蛋白表达与患者年龄、性别、肿块大小及病理类型均无相关性；与淋巴结转移、TNM分期呈显著相关。 ERCC1高表达患者预后差是影响患者预后的独立因素。 MTT结果显示在DDP敏感组中ERCC1阳性表达率明显高于DDP耐药组中ERCC1阳性表达率。结论 ERCC1是影响非小细胞肺癌预后的独立因素，同时又是提示DDP耐药的一个重要指标。
목적：탐토ERCC1재비소세포폐암중적표체급기림상의의。방법이용면역조화법검측ERCC1단백재160례비소세포폐암조직중표체정황，분석ERCC1단백표체여비소세포폐암림상병리삼수적관계급예후적상관성。동시류취저160례술중표본진행원대배양，검측기대DDP적약물민감성급여ERCC1표체적상관성。결과 ERCC1단백주요재세포핵중정현종황색과립。폐암조직ERCC1단백표체여환자년령、성별、종괴대소급병리류형균무상관성；여림파결전이、TNM분기정현저상관。 ERCC1고표체환자예후차시영향환자예후적독립인소。 MTT결과현시재DDP민감조중ERCC1양성표체솔명현고우DDP내약조중ERCC1양성표체솔。결론 ERCC1시영향비소세포폐암예후적독립인소，동시우시제시DDP내약적일개중요지표。
Objective To explore the expression of Excision repair cross -completion 1 in non-small cell lung cancer and to analyze its clinical significance .Methods ERCC1 protein expression was detected by IHC method in 160 cases of non-small cell lung cancer tissue .We analyzed relationship between the ERCC 1 ex-pression and clinicopathological parameters of non -small cell lung cancer ,as well as its correlation between ER-CC1 expression and the prognosis .At the same time ,160 cases of intraoperative specimens were primarily cul-tured.The relationship between ERCC1 expression and the drug sensitivity of DDP was analyzed .Results ER-CC1 protein was mainly expressed in the nucleus .ERCC1 protein expression in lung cancer tissue showed no rela-tionship with age,gender,tumor size and pathological types .ERCC1 protein expression was associated with TNM stages and lymph node metastases .High expression of ERCC1was significantly correlated with poor prognosis in pa-tients,indicating that ERCC1 is the independent factor of NSCLC patients .MTT results showed that the positive ex-pression rate of ERCC1 in DDP sensitive groups was higher than in DDP resistant groups .Conclusion ERCC1 is an independent factor influencing the prognosis of non -small cell lung cancer ,and associated with DDP resistance .