CAJ | 학술논문

目的探讨内皮素和一氧化氮在神经源性膀胱病人血浆和尿中含量的变化及其临床意义。方法将2003年10月至2004年8月明确诊断的22例患儿作为实验组，均为反射亢进型膀胱源性膀胱。留取空腹血和晨尿，采用酶联免疫方法检测血浆和尿中内皮素（ET—1），一氧化氮（NO）含量，选取同期入院年龄匹配的儿童的血尿标本（腹股沟斜和鞘膜积液30例）设为对照组。结果实验组22例，血浆 ET—1含量（0．487±0．039）fmol／mL，NO 含量（69．57±36．68）μmol／L，尿中 ET —1含量为（0.453±0．029）fmol／mL，NO 含量（392．36±121．1）μmol／L。对照组血浆 ET —1含量（0．494±0.117） fmol／mL，NO 含量（74．56±11．39）μmol／L，尿中 ET—1含量（0．548±0.073）fmol／mL，NO 含量（268．92±88．38）μmol／L。实验组中血浆 ET—1含量高于尿中 ET—1含量（P ＜0．01），尿中 ET—1含量低于对照组（P ＜0．05），尿中 NO 含量高于对照组（P ＜0．05）；血浆中 ET —1、NO 含量较对照组无明显差异（P ＞0.05）。结论反射亢进型神经源性膀胱病人血浆中 ET 和 NO 的含量处于正常水平；而尿中 ET—1和NO 比例失衡。
목적탐토내피소화일양화담재신경원성방광병인혈장화뇨중함량적변화급기림상의의。방법장2003년10월지2004년8월명학진단적22례환인작위실험조，균위반사항진형방광원성방광。류취공복혈화신뇨，채용매련면역방법검측혈장화뇨중내피소（ET—1），일양화담（NO）함량，선취동기입원년령필배적인동적혈뇨표본（복고구사화초막적액30례）설위대조조。결과실험조22례，혈장 ET—1함량（0．487±0．039）fmol／mL，NO 함량（69．57±36．68）μmol／L，뇨중 ET —1함량위（0.453±0．029）fmol／mL，NO 함량（392．36±121．1）μmol／L。대조조혈장 ET —1함량（0．494±0.117） fmol／mL，NO 함량（74．56±11．39）μmol／L，뇨중 ET—1함량（0．548±0.073）fmol／mL，NO 함량（268．92±88．38）μmol／L。실험조중혈장 ET—1함량고우뇨중 ET—1함량（P ＜0．01），뇨중 ET—1함량저우대조조（P ＜0．05），뇨중 NO 함량고우대조조（P ＜0．05）；혈장중 ET —1、NO 함량교대조조무명현차이（P ＞0.05）。결론반사항진형신경원성방광병인혈장중 ET 화 NO 적함량처우정상수평；이뇨중 ET—1화NO 비례실형。
Objective To explore the quantity of endothelin and nitrogen monoxidum in blood plasm and urinary and it's significance in neurogenic bladder diseases. Methods The 22 patients of neurogenic bladder were from the period during October 2003 to August 2004,10 months ~14 years old,the average was 6.28 ± 3.12 years old.All patients underwent urodynamic study,nervous electromyogram,voiding cysto urethro gra-phy and intravenous pyelography(IVP).Using zymo-immunity,we detected the contents of endothelin and ni-trogen monoxidum blood of empty stomach and urina sanguinis.And using chrome disoxidation biochemistry,we detected nitrogen monoxidum(NO)from blood and urinary,and control group age match 30 cases (oblique in-guinal hernia and funicular hydrocele). Results All patients suffered to hyperreflexia.The endothelin-1 in plasma was *0.487 ±0.039)fmol/mL,and in the urinary was (0.453 ±0.029)fmol/mL,NO in plasma was (69.57 ±36.68)μmol/L,and in the urinary was (392.36 ±121.1)μmol/L.In control group the endothelin-1 in plasma was (0.494 ±0.117)fmol/mL,and in the urinary was (0.548 ±0.073)fmol/mL.NO in plasma was (74.56 ±11.39)μmol/L,and in the urinary was (268.92 ±88.38)μmol/L .Endothein-1 in plasma was higher than in urinary(P <0.01),and NO in the urinary was higher than that of normal children(P <0.05).Endothelin-1 and NO of plasma in neurogenic bladders were not significant with normal children(P >0.05). Conclusion Endothelin and NO in plasma are at the normal level in hyperreflexia patients,but in the urinary lied disproportion of endothelin and NO,and endothelin is higher,and NO is lower than normal.