环境科技
環境科技
배경과기
ENVIRONMENTAL SCIENCE AND TECHNOLOGY
2014年
6期
5-8,13
,共5页
复垦矿区土壤%矿区复合基质%有机碳%酶活性%群落水平生理结构
複墾礦區土壤%礦區複閤基質%有機碳%酶活性%群落水平生理結構
복은광구토양%광구복합기질%유궤탄%매활성%군낙수평생리결구
Reclaimed mine soil%Composite substrate%Organic carbon%Enzyme activity%Community level physiological profile
以适当比例混合煤矸石、粉煤灰和活性污泥并种植黑麦草构建植物-矿区复合基质体系，测试复合基质有机碳及酶活性变化，利用群落水平生理结构（CLPP）对复合基质微生物功能多样性进行解析，分析了矿区复合基质中有机碳动态变化及其与酶活性、微生物多样性关系。结果表明：植物-矿区复合基质体系3种基质中有机碳随复垦年限增长而增加，且在第3年煤矸石+粉煤灰+污泥（F+G+S）区有机碳达到最大，分别是煤矸石+粉煤灰（F+G）区和对照区的1.1和1.2倍。3个复垦区域复合基质中蔗糖酶、脱氢酶、酸性磷酸酶酶活性随复垦时间增长而增加。微生物多样性CLPP解析结果表明，微生物种类在复垦1年时最多，微生物优势度和均一性随复垦时间逐渐变大；同时3个区域基质微生物多样性与营养元素相关性不大，可能有机碳可获得性低成为微生物生长的限制因子。
以適噹比例混閤煤矸石、粉煤灰和活性汙泥併種植黑麥草構建植物-礦區複閤基質體繫，測試複閤基質有機碳及酶活性變化，利用群落水平生理結構（CLPP）對複閤基質微生物功能多樣性進行解析，分析瞭礦區複閤基質中有機碳動態變化及其與酶活性、微生物多樣性關繫。結果錶明：植物-礦區複閤基質體繫3種基質中有機碳隨複墾年限增長而增加，且在第3年煤矸石+粉煤灰+汙泥（F+G+S）區有機碳達到最大，分彆是煤矸石+粉煤灰（F+G）區和對照區的1.1和1.2倍。3箇複墾區域複閤基質中蔗糖酶、脫氫酶、痠性燐痠酶酶活性隨複墾時間增長而增加。微生物多樣性CLPP解析結果錶明，微生物種類在複墾1年時最多，微生物優勢度和均一性隨複墾時間逐漸變大；同時3箇區域基質微生物多樣性與營養元素相關性不大，可能有機碳可穫得性低成為微生物生長的限製因子。
이괄당비례혼합매안석、분매회화활성오니병충식흑맥초구건식물-광구복합기질체계，측시복합기질유궤탄급매활성변화，이용군낙수평생리결구（CLPP）대복합기질미생물공능다양성진행해석，분석료광구복합기질중유궤탄동태변화급기여매활성、미생물다양성관계。결과표명：식물-광구복합기질체계3충기질중유궤탄수복은년한증장이증가，차재제3년매안석+분매회+오니（F+G+S）구유궤탄체도최대，분별시매안석+분매회（F+G）구화대조구적1.1화1.2배。3개복은구역복합기질중자당매、탈경매、산성린산매매활성수복은시간증장이증가。미생물다양성CLPP해석결과표명，미생물충류재복은1년시최다，미생물우세도화균일성수복은시간축점변대；동시3개구역기질미생물다양성여영양원소상관성불대，가능유궤탄가획득성저성위미생물생장적한제인자。
The plant-mining complex substrate system was carried out by appropriate proportion of mixed coal gangue,fly ash and activated sludge, at the same time perennial ryegrass were also planted. The organic carbon and enzymatic activity of composite substrate was tested and the microbial diversity was analyzed by using community level physiological profile (CLPP). The objective of this paper was to explore the dynamic changes of organic carbon and its relation with enzymatic activity and microbial diversity. Results show that organic carbon in three kinds of complex substrates of plant-mining complex substrate system increased with reclamation time. At the 3rd year of reclamation, the amount of organic carbon in area mixed with fly ash, coal gangue and sludge (F+G+S) reached the maximum, which is 1.1 and 1.2 times than the area of fly ash+coal gangue (F+G) and control respectively. The enzyme activity of sucrase, dehydrogenase and acid phosphatase increases with reclamation time in three composite substrate reclamation areas. Microbial diversity analytical results showed that the microbial diversity in the first year ofreclamation was the highest, while the Simpson and McIntosh indexes grow larger over time. Meanwhile, the microbial diversity was related to soil nutrients;the reason may be that the low level of SOC restricts the growth of microbes.
