CAJ | 학술논문

目的：检测高迁移率族蛋白B1（HMGB1）在胃癌组织的表达，分析其与胃癌临床病理特征的关系。方法112例胃癌及癌旁组织标本应用免疫组织化学法、反转录 PCR、Western‐blot检测 HMGB1的表达、定量，应用统计学方法分析其与胃癌临床病理特征关系。结果免疫组织化学染色显示：HMGB1阳性颗粒主要位于细胞核；反转录 PCR、Western‐blot显示：胃癌组织中的表达水平明显高于癌旁组织和正常胃组织（P＜0．01），HMGB1过度表达与胃癌的分化程度、组织学的类型及有无淋巴结转移关系密切。结论 HMGB1在胃癌组织中高表达与胃癌的临床病理特征密切相关，推测其过表达可能与胃癌的侵袭、转移有关。
목적：검측고천이솔족단백B1（HMGB1）재위암조직적표체，분석기여위암림상병리특정적관계。방법112례위암급암방조직표본응용면역조직화학법、반전록 PCR、Western‐blot검측 HMGB1적표체、정량，응용통계학방법분석기여위암림상병리특정관계。결과면역조직화학염색현시：HMGB1양성과립주요위우세포핵；반전록 PCR、Western‐blot현시：위암조직중적표체수평명현고우암방조직화정상위조직（P＜0．01），HMGB1과도표체여위암적분화정도、조직학적류형급유무림파결전이관계밀절。결론 HMGB1재위암조직중고표체여위암적림상병리특정밀절상관，추측기과표체가능여위암적침습、전이유관。
Objective To investigate the significance of high mobility group protein B1 (HMGB1) in gastric carcinoma (GC) and its relationship with clinicopathological characteristics .Methods 112 cases of GC specimens and tumor‐adjacent tissues were collected .The expression of HMGB1 was tested with immunohistochemistry ,re‐verse transcription‐polymerase chain reaction (RT‐PCR ) and Western‐blot .Clinicopathological characteristics of GC patients were also analysed .The relationship between HMGB1 and the pathological characteristics of GC was analysed statistically .Results Immunohistochemistry data demonstrated that HMGB1 protein was predominantly localized in the nuclei of GC .Results of RT‐PCR and Western‐blot demonstrated that the expression of relative HMGB1 mRNA and protein in the tissue of GC were higher than that in tissue adjacent to carcinoma and normal gastric tissue ( P<0 .01 ) .Overexpression of HMGB1 was significantly correlated with histological type ,differentia‐tion and lymph node metastasis of tumor .Conclusion Overexpression of HMGB1 was related to some clinicopatho‐logical characteristics of GC ,and HMGB1 may promote invasion and metastasis of GC .