CAJ | 학술논문

目的：研究大黄乙醇提取物体外抗麻疹病毒的作用及其途径。方法采用组织细胞培养法，使用麻疹病毒野毒株、标准株和疫苗株，采用抗病毒生物合成组、直接杀灭组、预防作用组3种加药方式，在Vero‐SLAM细胞上观察大黄乙醇提取物抗麻疹病毒作用。结果大黄乙醇提取物在Vero‐SLAM 细胞上对不同来源的麻疹病毒株均具有明显的抗病毒生物合成和直接杀灭作用，药物的最小有效浓度（M IC ）为156μg／ml ，最大无毒浓度（0％ toxility concentration ，TC0）为5000μg／ml ，治疗指数（ TI ）为32。结论大黄乙醇提取物在体外对麻疹病毒具有显著抗病毒作用，安全性较高，应用前景广泛。
목적：연구대황을순제취물체외항마진병독적작용급기도경。방법채용조직세포배양법，사용마진병독야독주、표준주화역묘주，채용항병독생물합성조、직접살멸조、예방작용조3충가약방식，재Vero‐SLAM세포상관찰대황을순제취물항마진병독작용。결과대황을순제취물재Vero‐SLAM 세포상대불동래원적마진병독주균구유명현적항병독생물합성화직접살멸작용，약물적최소유효농도（M IC ）위156μg／ml ，최대무독농도（0％ toxility concentration ，TC0）위5000μg／ml ，치료지수（ TI ）위32。결론대황을순제취물재체외대마진병독구유현저항병독작용，안전성교고，응용전경엄범。
Ob j ective To investigate the anti‐viral effects of rhubarb ethanol extract on measles virus infec‐tion in vitro .Methods Cytopathic effect(CPE) were used to evaluate the toxicity of rhubarb ethanol extract a‐gainst V ero‐SLAM cells .The anti‐viral effects of rhubarb ethanol extract on measles virus infection in vitro was observed in three experimental groups :direct effect group ,anti‐biosynthesis group ,and precaution group .Results Rhubarb ethanol extract showed remarkable inhibitory and killing effects on measles virus .The MIC of rhubarb ethanol extract was 156μg/ml while the TC0 was 5000μg/ml .So therapeutic index(TI) was 32 .Conclusion It concluded that rhubarb ethanol extract showed strong antivirus effect on measles virus with high safety .