CAJ | 학술논문

目的：研究DNA 与药物分子的相互作用机理。方法利用等温滴定微量热法（ITC）、紫外吸收光谱法（UV）和荧光光谱法研究了亚叶酸钙（CaFL）与小牛胸腺脱氧核糖核酸（ct‐DNA）在缓冲溶液中的结合反应。结果当药物分子与2类结合位点相结合时，标准吉布斯自由能（ΔG1°和ΔG2°）的变化几乎是一样的，然而，标准焓变（ΔH1°和ΔH 2°）却不相同，第1类结合发生在磷酸基团上，以焓驱动为主，而第2类结合则主要发生在碱基对形成的位点上，并显示为熵驱动为主的过程。结论热力学结果表明，ct‐DNA大分子上存在可结合FL2－阴离子的2类位点。
목적：연구DNA 여약물분자적상호작용궤리。방법이용등온적정미량열법（ITC）、자외흡수광보법（UV）화형광광보법연구료아협산개（CaFL）여소우흉선탈양핵당핵산（ct‐DNA）재완충용액중적결합반응。결과당약물분자여2류결합위점상결합시，표준길포사자유능（ΔG1°화ΔG2°）적변화궤호시일양적，연이，표준함변（ΔH1°화ΔH 2°）각불상동，제1류결합발생재린산기단상，이함구동위주，이제2류결합칙주요발생재감기대형성적위점상，병현시위적구동위주적과정。결론열역학결과표명，ct‐DNA대분자상존재가결합FL2－음리자적2류위점。
Objective To study the interaction mechanism of DNA with drug .Methods The interaction of calcium folinate(CaFL) with calf thymus deoxyribonucleic acid (ct DNA) in aqueous buffer solution had been in‐vestigated by isothermal titration calorimetry (ITC ) ,ultraviolet absorption (UV ) and fluorescence spectroscopy . Results TherewasonlyasubtledifferencebetweenchangesinstandardGibbsfreeenergy(ΔG1°andΔG2°),cor‐responding to the first and the second classes of binding processes .But the changes of standard enthalpy (ΔH1°andΔH2°) were evidently different .One class of binding took place on phosphate groups ,which was driven by enthal‐py ,while the other one took place at the sites formed by base pairs and appeared as an entropy driven process .The thermodynamic behavior of the DNA‐drug supramolecular system had been discussed according to the spectra in‐formation .Conclusion Analysis of the thermodynamic data indicates that there are two classes of binding sites on the DNA molecules being able to coordinate with FL 2 -anions .