CAJ | 학술논문

探讨 PFOS 对雄性小鼠睾丸细胞凋亡以及相关基因表达的影响。40只雄性昆明系小鼠随机分成 PFOS 0、1.25、2.5、5.0和10.0 mg/kg，共5组，通过饮水方法染毒35天。处死小鼠，取睾丸，流式细胞仪观察细胞凋亡 RT-PCR 观察 Bax 和 Bcl-2基因表达。细胞凋亡率随着 PFOS 的浓度增加而增加，各剂量组与对照组比较均有显著性差异；10 mg/kg/d PFOS 实验组小鼠睾丸组织中 Bax 基因表达与对照组比较显著上升（p<0.05），其他剂量组变化与对照组比较差异无统计学意义（p>0.05）。随着染毒剂量的增加，Bcl-2基因表达下降，5.0、10.0 mg/kg/d PFOS 实验组与对照组相比差异有统计学意义（分别为 p<0.05，p<0.01）PFOS 能够引起睾丸细胞凋亡增加，Bax 和 Bcl 基因表达改变可能是其机制之一。
탐토 PFOS 대웅성소서고환세포조망이급상관기인표체적영향。40지웅성곤명계소서수궤분성 PFOS 0、1.25、2.5、5.0화10.0 mg/kg，공5조，통과음수방법염독35천。처사소서，취고환，류식세포의관찰세포조망 RT-PCR 관찰 Bax 화 Bcl-2기인표체。세포조망솔수착 PFOS 적농도증가이증가，각제량조여대조조비교균유현저성차이；10 mg/kg/d PFOS 실험조소서고환조직중 Bax 기인표체여대조조비교현저상승（p<0.05），기타제량조변화여대조조비교차이무통계학의의（p>0.05）。수착염독제량적증가，Bcl-2기인표체하강，5.0、10.0 mg/kg/d PFOS 실험조여대조조상비차이유통계학의의（분별위 p<0.05，p<0.01）PFOS 능구인기고환세포조망증가，Bax 화 Bcl 기인표체개변가능시기궤제지일。
To detect the effect of PFOS on the apoptosis and the express of Bax and Bcl-2 in testes of male mice. 40 male mice were divided into five groups, and were exposed to 0, 1.25, 2.5, 5.0 和 10.0 mg/kg PFOS. After 35 days, mice were sufficed and testes were tested by flow cytometry and the gene express of Bcl-2 and Bax were detected by RT-PCR. The rate of apoptosis increased with the increase of PFOS and there is a significant difference between the PFOS-treated groups and the control group. The gene express of Bax of 10mg/kg PFOS is significantly different from the control group (p<0.05) and there is no difference between other group and the control group (p>0.05).The gene express of Bcl-2 increased with the increase of PFOS. There is significant difference in group of 5 mg/kg and 10mg/kg PFOS compared to the control group. PFOS can induce the increase of apoptosis of testes and the effect of PFOS on the express of Bcl-2 and Bax gene may be the one of mechanisms.