CAJ | 학술논문

谷胱甘肽过氧化酶2(GPx2)，是生物体内重要的一类抗氧化酶，能够通过酶解过氧化氢阻断活性氧(ROS)的有害作用。本研究从七鳃鳗的血液 cDNA 文库中克隆了 GPx2 cDNA 全长和基因组 DNA 序列。序列分析表明，其 cDNA全长为868 bp(GenBank 序列号 KM211710)，包括53bp 5′非编码区、251bp 3′非编码区和564 bp 开放阅读框，编码由187个氨基酸组成的多肽。基因组 DNA 序列(GenBank 序列号 KM211711)揭示了基因组特征含有2个外显子和1个内含子结构。系统发育分析表明，七鳃鳗 GPx2(LjGPx2)与其它的 GPx2具有很高的同源性。利用实时荧光定量 PCR检测到 GPx2基因在七鳃鳗各组织中广泛表达，其中在口腔腺、心脏、卵、生殖腺中表达量较高。此外，用脂多糖(LPS)体内刺激七鳃鳗后发现，LjGPx2 mRNA 在生殖腺中表达量显著升高。本研究为探讨 GPx2在七鳃鳗的免疫应答中的作用提供了理论依据。
곡광감태과양화매2(GPx2)，시생물체내중요적일류항양화매，능구통과매해과양화경조단활성양(ROS)적유해작용。본연구종칠새만적혈액 cDNA 문고중극륭료 GPx2 cDNA 전장화기인조 DNA 서렬。서렬분석표명，기 cDNA전장위868 bp(GenBank 서렬호 KM211710)，포괄53bp 5′비편마구、251bp 3′비편마구화564 bp 개방열독광，편마유187개안기산조성적다태。기인조 DNA 서렬(GenBank 서렬호 KM211711)게시료기인조특정함유2개외현자화1개내함자결구。계통발육분석표명，칠새만 GPx2(LjGPx2)여기타적 GPx2구유흔고적동원성。이용실시형광정량 PCR검측도 GPx2기인재칠새만각조직중엄범표체，기중재구강선、심장、란、생식선중표체량교고。차외，용지다당(LPS)체내자격칠새만후발현，LjGPx2 mRNA 재생식선중표체량현저승고。본연구위탐토 GPx2재칠새만적면역응답중적작용제공료이론의거。
Glutathione peroxidase 2 (GPx2) is an important antioxidant enzyme in vivo that enzymatically reduces hydrogen peroxide to limit harmful effects of reactive oxygen species (ROS) in living organisms. In this study, the full-length GPx2 gene and the genomic DNA sequences were cloned from the blood cDNA library of Lampetra japonica (L. japonica). The sequence analysis showed that the full-length cDNA of 868 bp (GenBank accession no. KM211710) includes an open reading frame (ORF) of 564 bp plus 53 bp of the 5'-untranslated region (5'UTR) and 251 bp of the 3'-untranslated region (3'UTR). Genomic DNA sequences (GenBank accession no. KM211711) included a two exon-one intron structure. Phylogenetic analysis revealed that GPx2 (LjGPx2) is closely related to other GPx2. The real-time PCR results demonstrated that the GPx2 gene was widely expressed in adult lamprey in which higher levels in buccal glands, heart, ovum, and gonad. Furthermore, upon in vivo stimulation of lampreys with lipopolysaccharide (LPS), LjGPx2 mRNA was upregulated in gonad. The results obtained in the present study will help us to understand the roles of GPx2 in immune responses in jawless vertebrates.