CAJ | 학술논문

目的：按照修订的硫代硫酸盐-柠檬酸盐-胆盐-蔗糖(TCBS)琼脂培养基行业标准中的要求进行试验,验证该行业标准的适用性。方法取不同厂家生产的TCBS琼脂培养基,根据TCBS琼脂培养基行业标准的要求,进行pH值、水分的测定和微生物生长试验。测定了TCBS琼脂培养基的pH值和水分,并将质控菌株的培养物接种到受试的培养基平皿中进行微生物生长试验。结果TCBS琼脂培养基的pH值和水分均符合行业标准的规定,且各质控菌株生长良好。结论作为推荐性的国家行业标准,TCBS琼脂培养基行业标准可以用于我国该培养基的质量评价和监管工作的需要。
목적：안조수정적류대류산염-저몽산염-담염-자당(TCBS)경지배양기행업표준중적요구진행시험,험증해행업표준적괄용성。방법취불동엄가생산적TCBS경지배양기,근거TCBS경지배양기행업표준적요구,진행pH치、수분적측정화미생물생장시험。측정료TCBS경지배양기적pH치화수분,병장질공균주적배양물접충도수시적배양기평명중진행미생물생장시험。결과TCBS경지배양기적pH치화수분균부합행업표준적규정,차각질공균주생장량호。결론작위추천성적국가행업표준,TCBS경지배양기행업표준가이용우아국해배양기적질량평개화감관공작적수요。
Objective To verify the application of professional standard for Thiosulfate-Citrate-Bile Salts-Sucrose (TCBS)agar medium.Methods TCBS agar medium produced by different factories are obtained for pH value and microbiological grow test according to the formulated medium standard.pH value and solution of medium are determined and bacterial cultures of the control strains are inoculated in the medium to detected bacterial growth.Results pH value and solution of medium were within the specified range. The control strains grew well. Conclusion As the recommended industrial standard, TCBS agar medium standard may be applied to evaluate and supervise the TCBS agar medium quality in our country.