中国药事
中國藥事
중국약사
CHINESE PHARMACEUTICAL AFFAIRS
2014年
12期
1357-1360
,共4页
张韶辉%郭珩%邓体瑛%徐宏峰%张耕
張韶輝%郭珩%鄧體瑛%徐宏峰%張耕
장소휘%곽형%산체영%서굉봉%장경
多柔比星%二苯乙烯苷%凋亡%线粒体膜电位
多柔比星%二苯乙烯苷%凋亡%線粒體膜電位
다유비성%이분을희감%조망%선립체막전위
doxorubicin%2,3,5,4 ’-tetrah-ydroxystilbene-2-O-β-D-glucoside%apoptosis%mitochondrial membrane potential
目的：研究中药何首乌主要有效成分二苯乙烯苷(THSG)对多柔比星(DOX)致心肌细胞凋亡的保护作用及机制。方法分离培养乳鼠心肌细胞，观察 DOX 单独及与 THSG 联合处理对心肌细胞的凋亡、线粒体膜电位及凋亡相关蛋白表达的影响。结果 THSG能够剂量依赖性地减少DOX诱导的心肌细胞凋亡、线粒体膜电位下降、抑制caspase-3激活、增加Bcl-2及减少Bax的蛋白表达。结论 THSG对DOX诱导的心肌细胞凋亡具有保护作用，且作用与其能够减少线粒体膜电位下降、抑制 caspase-3激活，增加Bcl-2及减少Bax的蛋白表达有关。
目的：研究中藥何首烏主要有效成分二苯乙烯苷(THSG)對多柔比星(DOX)緻心肌細胞凋亡的保護作用及機製。方法分離培養乳鼠心肌細胞，觀察 DOX 單獨及與 THSG 聯閤處理對心肌細胞的凋亡、線粒體膜電位及凋亡相關蛋白錶達的影響。結果 THSG能夠劑量依賴性地減少DOX誘導的心肌細胞凋亡、線粒體膜電位下降、抑製caspase-3激活、增加Bcl-2及減少Bax的蛋白錶達。結論 THSG對DOX誘導的心肌細胞凋亡具有保護作用，且作用與其能夠減少線粒體膜電位下降、抑製 caspase-3激活，增加Bcl-2及減少Bax的蛋白錶達有關。
목적：연구중약하수오주요유효성분이분을희감(THSG)대다유비성(DOX)치심기세포조망적보호작용급궤제。방법분리배양유서심기세포，관찰 DOX 단독급여 THSG 연합처리대심기세포적조망、선립체막전위급조망상관단백표체적영향。결과 THSG능구제량의뢰성지감소DOX유도적심기세포조망、선립체막전위하강、억제caspase-3격활、증가Bcl-2급감소Bax적단백표체。결론 THSG대DOX유도적심기세포조망구유보호작용，차작용여기능구감소선립체막전위하강、억제 caspase-3격활，증가Bcl-2급감소Bax적단백표체유관。
Objective To investigate the protective effect and mechanism of 2,3,5,4’-tetrah-ydroxystilbene-2-O-β-D-glucoside (THSG)on cardiomyocyte apoptosis induced by doxorubicin (DOX). Methods The neonatal rats’cardiomyocytes were isolated and cultured.The effect of DOX alone and DOX combined treatment with THSG on apoptosis,mitochondrial membrane potential (MMP)and apoptosis-related proteins expression were observed.Results THSG could dose-dependently ameliorate DOX-induced apoptosis and the loss of MMP,inhibit caspase-3 activation,increase the expression of Bax protein and reduce the expression of Bcl-2 protein, respectively. Conclusion THSG could ameliorate the cardiomyocyte apoptosis induced by DOX involving alleviating loss of MMP, caspase-3 activation and changes of Bax protein expression.
