CAJ | 학술논문

目的：：评价槲皮素对前列腺癌PC-3细胞凋亡和热休克蛋白27( HSP27)表达的影响，探讨槲皮素抗前列腺癌的可能作用机制。方法：体外培养人前列腺癌RWPE-1、 LNCaP、 PC-3和TSU-Pr1细胞株，分别随机分为4组：空白对照组；阴性对照组：给予二甲亚砜(DMSO)；给药高剂量组：给予0．3mg/mL槲皮素150μL；给药低剂量组：给予0．6mg/mL槲皮素150μL。以TUNEL法检测给予槲皮素后PC-3细胞凋亡、 RT-PCR检测HSP27的表达情况。结果：给药组可见大量PC-3细胞凋亡，且呈剂量依赖性，与空白对照组比较，差异具有统计学意义(P<0．05)；给药组PC-3细胞HSP27的表达明显降低，且呈剂量依赖性，与空白对照组比较，差异具有统计学意义( P<0．05)。结论：槲皮素可诱导前列腺癌PC-3细胞凋亡和抑制HSP27的表达，可能是其抗前列腺癌的机制之一。
목적：：평개곡피소대전렬선암PC-3세포조망화열휴극단백27( HSP27)표체적영향，탐토곡피소항전렬선암적가능작용궤제。방법：체외배양인전렬선암RWPE-1、 LNCaP、 PC-3화TSU-Pr1세포주，분별수궤분위4조：공백대조조；음성대조조：급여이갑아풍(DMSO)；급약고제량조：급여0．3mg/mL곡피소150μL；급약저제량조：급여0．6mg/mL곡피소150μL。이TUNEL법검측급여곡피소후PC-3세포조망、 RT-PCR검측HSP27적표체정황。결과：급약조가견대량PC-3세포조망，차정제량의뢰성，여공백대조조비교，차이구유통계학의의(P<0．05)；급약조PC-3세포HSP27적표체명현강저，차정제량의뢰성，여공백대조조비교，차이구유통계학의의( P<0．05)。결론：곡피소가유도전렬선암PC-3세포조망화억제HSP27적표체，가능시기항전렬선암적궤제지일。
!O!b!jec"tive !!"To observe the effect of quereetin on prostate cancer PC-3 cell apoptosis and heat shock protein 27 (HSP27) expression, and discuss therapeutic mechanism of quereetin. !M!e!tho"d ! !"RT-PCR of the HSP27 was implemented by the culture of RWPE-1, LNCaP, PC-3, and TSU-Pr1 cell lines. Analysis was separately conducted in the control group, control vector group treated by dimethyl sulfoxide (DMSO), and groups treated with 150μL quereetin at 0. 3 or 0. 6mg/mL concentration. PC-3 cell apoptosis was evaluated by TUNEL staining, and the expression of HSP27 was observed in RT-PCR. !!Re!su"lt !!"After quereetin treatment, the number of PC-3 cell apoptosis at 0. 6mg/mL quereetin concentration was stronger compared to that at 0. 3mg/mL concentration. Compared with the control group, the difference was statistically significant (P<0. 05) . Compared with the control group, the HSP27 expression detected by RT-PCR was stronger at 0. 6mg/mL quereetin concentration compared to that at 0. 3mg/mL concentration, and the difference was statistically significant (P<0. 05) . !C!on!cl"usion !!!"Quereetin could induce apoptosis in PC-3 and inhibit HSP27 expression, which might be one mechanism of its anti-prostate cancer.