CAJ | 학술논문

为了探寻一种高效、稳定、廉价的桑蟥基因组DNA提取方法，通过采用CTAB法、蛋白酶K法和盐析法3种方法研究桑蟥基因组DNA的提取。对提取的DNA进行分光光度值的测定以及COI基因的扩增鉴定。结果表明：3种方法均可以提取基因组DNA，蛋白酶K法和CTAB法DNA获得率优于饱和NaCl法提取的DNA，3种方法提取的DNA都可用于PCR扩增实验。因此，在实际工作中可根据实验目的、条件选取最适合的方法。
위료탐심일충고효、은정、렴개적상황기인조DNA제취방법，통과채용CTAB법、단백매K법화염석법3충방법연구상황기인조DNA적제취。대제취적DNA진행분광광도치적측정이급COI기인적확증감정。결과표명：3충방법균가이제취기인조DNA，단백매K법화CTAB법DNA획득솔우우포화NaCl법제취적DNA，3충방법제취적DNA도가용우PCR확증실험。인차，재실제공작중가근거실험목적、조건선취최괄합적방법。
To explore an efficient, stable, low-cost DNA extraction method from Rondotia menciana Moore, genomic DNA was extracted using CTAB, protein enzyme K and salting-out method, respectively. The extracted genomic DNA samples of three methods were tested using photometric analysis and COI gene amplification. The results showed that, all the three methods can be used for the total DNA extraction, which are suitable for the PCR amplification, but genome DNA extracted by CTAB method and protein enzyme K method were higher in purity and production, degraded slightly. So we should select the proper method according to the objective and condition in the study.