CAJ | 학술논문

以意大利苍耳叶为原料，研究其总酚的提取条件，同时对意大利苍耳叶总酚提取物的抗氧化活性进行评价。选用乙醇-水混合物为溶剂，采用超声辅助提取法处理意大利苍耳叶。将中心组合实验设计原理和响应面分析方法相结合，模拟得到二元多次相回归方程的预测模型和可信度，并通过岭脊分析方法得到最佳提取条件。使用福林酚试剂对意大利苍耳叶的总酚含量进行测定。分别采用铁离子还原法、DPPH 自由基清除法测定意大利苍耳叶提取物的抗氧化能力。结果表明：超声波辅助提取意大利苍耳叶总酚的最佳工艺条件为乙醇体积分数54％、超声时间31 min、液固比195 mL·g－1；意大利苍耳叶总酚提取率为（3．12±0．27）％。意大利苍耳叶总酚提取液对铁离子还原的 IC50为（774．5±7．26）mg·L－1，对 DPPH 自由基的半数抑制率IC50为（250．9±5．48）mg·L－1。由响应面法优化得到的意大利苍耳叶总酚的提取工艺方便可行，得到的意大利苍耳叶总酚具有一定抗氧化活性。
이의대리창이협위원료，연구기총분적제취조건，동시대의대리창이협총분제취물적항양화활성진행평개。선용을순-수혼합물위용제，채용초성보조제취법처리의대리창이협。장중심조합실험설계원리화향응면분석방법상결합，모의득도이원다차상회귀방정적예측모형화가신도，병통과령척분석방법득도최가제취조건。사용복림분시제대의대리창이협적총분함량진행측정。분별채용철리자환원법、DPPH 자유기청제법측정의대리창이협제취물적항양화능력。결과표명：초성파보조제취의대리창이협총분적최가공예조건위을순체적분수54％、초성시간31 min、액고비195 mL·g－1；의대리창이협총분제취솔위（3．12±0．27）％。의대리창이협총분제취액대철리자환원적 IC50위（774．5±7．26）mg·L－1，대 DPPH 자유기적반수억제솔IC50위（250．9±5．48）mg·L－1。유향응면법우화득도적의대리창이협총분적제취공예방편가행，득도적의대리창이협총분구유일정항양화활성。
Using the leaves of X.italicum Moretti as raw material,the extraction conditions of total phenols and antioxidant ac-tivity of the total phenolic extract was investiged.Using ethanol-water mixture as solvent,the leaves of X.italicum Moretti were processed by ultrasonic assisted extraction.The center combination experiment design principle and the analysis of the response surface method were combined to get the forecast model of binary regression equation of the multiple phase and credibility,and the best extraction conditions were obtained through ridge analysis method.The total phenolic content was determined by the Fo-lin-phenol reagent.Antioxidant capacity was measured using iron reduction method and DPPH radical scavenging method respec-tively.The results show that the optimum conditions of ultrasonic assisted extraction are ethanol concentration 54%,extraction time 31 min,liquid-solid ratio 195 mL·g-1 ,and yield of total phenols is (3.12±0.27)%.The leaves of X.italicum Moretti on the reduction of iron ions IC50 was (774.5±7.26)mg·L-1 and DPPH IC50 is (250.9±5.48)mg·L-1 .The response surface method to optimize the extraction process of total phenols are convenient and feasible,and the X.italicum Moretti leaves total phenol has certain antioxidant activity.