CAJ | 학술논문

为了研究肥胖基因 FTO 过表达对小鼠胰岛β细胞功能的影响以及基因表达谱的变化，构建小鼠 FTO 基因过表达慢病毒载体，包装慢病毒颗粒并感染小鼠胰岛 MIN6细胞。利用 QPCR 和 Western Blot 技术检测 FTO 基因在 MIN6细胞中过表达情况，并检测葡萄糖刺激检测胰岛素的释放情况，进一步利用小鼠全基因芯片检测 FTO 过表达对小鼠胰岛 MIN6细胞表达谱的影响。结果表明：慢病毒载体成功介导了 FTO 在 MIN6细胞中过表达，FTO 过表达可以显著抑制 MIN6细胞的胰岛素释放。表达芯片的结果显示 FTO 改变 MIN6细胞表达谱，发现多达922个的差异基因。FTO 过表达改变了小鼠胰岛细胞的表达谱，差异基因通过一些重要信号通路影响小鼠胰岛β细胞的生物学功能。
위료연구비반기인 FTO 과표체대소서이도β세포공능적영향이급기인표체보적변화，구건소서 FTO 기인과표체만병독재체，포장만병독과립병감염소서이도 MIN6세포。이용 QPCR 화 Western Blot 기술검측 FTO 기인재 MIN6세포중과표체정황，병검측포도당자격검측이도소적석방정황，진일보이용소서전기인심편검측 FTO 과표체대소서이도 MIN6세포표체보적영향。결과표명：만병독재체성공개도료 FTO 재 MIN6세포중과표체，FTO 과표체가이현저억제 MIN6세포적이도소석방。표체심편적결과현시 FTO 개변 MIN6세포표체보，발현다체922개적차이기인。FTO 과표체개변료소서이도세포적표체보，차이기인통과일사중요신호통로영향소서이도β세포적생물학공능。
To explore the effect of FTO (fat mass and obesity associated)overexpression on mouse pancreatic islet βcells and genes expression profile,mouse FTO gene was cloned into the lentivirus.Lentivirus was packaged in 293T cells and delivered in-to the pancreatic islet cells.QPCR and Western blot assay were employed to detect the overexpression of FTO in MIN6 cells. Glucose-stimulated insulin secretion (GSIS)was performed to detect the insulin secretion of MIN6 cells.Mouse gene microarray was used to detect the differential gene expression profile of the islet cells modulated by FTO overexpression.MIN6 cells stably expressing FTO was achieved by lentivirus delivery.FTO overexpression significantly inhibits the insulin secretion.The microar-ray shows that there are totally 922 differential expression genes.FTO overexpression changes the gene expression profile of MIN6 cells.The differentially expressed genes may regulate the isletβcells functions via important signal pathways.