CAJ | 학술논문

为载体与喹诺酮类抗生素耐药性之间的关系，采用 K-B 纸片法及微孔稀释法确定细菌对抗生素的药物敏感性，使用CAS 琼脂法和紫外可见分光光度法分别对肺炎克雷伯菌铁载体进行定性和定量检测，使用统计学软件 SPSS 分析细菌铁载体与其耐药性的关系。CAS 琼脂法分析表明，临床分离的70株肺炎克雷伯菌均产铁载体（100％），紫外分光光度法分析表明肺炎克雷伯菌中铁载体产量不同。根据统计学取中位数的方法能够将细菌分为铁载体高产组和低产组两组，喹诺酮类耐药株中铁载体产量高于敏感株（P＜0．01）；铁载体高产组对环丙沙星（CIP）和左氧氟沙星（LEV）的耐药率高于低产组（P ＜0．01）；肺炎克雷伯菌铁载体与细菌对喹诺酮类抗生素的耐药性呈正相关性（P＜0．01）。研究表明细菌铁载体参与了细菌耐药，干预了抗生素的杀菌过程。
위재체여규낙동류항생소내약성지간적관계，채용 K-B 지편법급미공희석법학정세균대항생소적약물민감성，사용CAS 경지법화자외가견분광광도법분별대폐염극뢰백균철재체진행정성화정량검측，사용통계학연건 SPSS 분석세균철재체여기내약성적관계。CAS 경지법분석표명，림상분리적70주폐염극뢰백균균산철재체（100％），자외분광광도법분석표명폐염극뢰백균중철재체산량불동。근거통계학취중위수적방법능구장세균분위철재체고산조화저산조량조，규낙동류내약주중철재체산량고우민감주（P＜0．01）；철재체고산조대배병사성（CIP）화좌양불사성（LEV）적내약솔고우저산조（P ＜0．01）；폐염극뢰백균철재체여세균대규낙동류항생소적내약성정정상관성（P＜0．01）。연구표명세균철재체삼여료세균내약，간예료항생소적살균과정。
To explore the relationship between siderophores and quinolone resistance in bacterial,K-B disk diffusion method and microporous dilution method were used in determination of antimicrobial susceptibility in the clinical isolates.CAS agar and UV-visible spectrophotometric assay were used for qualitative and quantifiability detections of siderophore.Association between pro-duction of siderophores and quinolone resistance were statistically analyzed by use of SPSS.According to CAS agar plate assay, 70 clinical isolates of Klebsiella pneumoniae all produce siderophores,however the production of siderophores measured by using UV-visible spectrometric method were different.Isolates were grouped into high siderophore-yielding group and low siderophore-yielding group based on median number.Siderophores in resistant strains of quinolone were significantly higher than the sensitive strains (P <0.01).The rate of ciprofloxacin and levofloxacin resistance in Klebsiella pneumoniae from the high siderophore-yielding group were significantly higher than those in low siderophore-yielding group (P<0.01).Siderophore in Klebsiella pneu-moniae was positively correlated with bacterial resistance to quinolones (P<0.01).The results show that siderophores involved in bacterial resistance and interfere the sterilization process of antibiotics.