CAJ | 학술논문

愈伤组织形成是植物组织培养的重要阶段。钙调素含量在愈伤组织形成过程中发生变化，但钙调素是否参与愈伤组织形成仍不清楚。为此，本文分析了一个钙调素基因CAM5的组织表达模式，结果发现，CAM5基因在拟南芥植株的根、茎、叶、花及幼苗中均有表达。此外，鉴定了拟南芥CAM5基因的纯合突变体 cam5，并观察其叶柄外植体在愈伤组织诱导过程中的表型，结果发现，在愈伤诱导培养基中培养20 d，与野生型相比，突变体的叶柄外植体膨大比较明显。进一步分析显示，培养20 d 后，突变体外植体的鲜重比培养前增加了39倍，而野生型仅增加了16倍。这些结果暗示CAM5基因参与了愈伤组织形成。
유상조직형성시식물조직배양적중요계단。개조소함량재유상조직형성과정중발생변화，단개조소시부삼여유상조직형성잉불청초。위차，본문분석료일개개조소기인CAM5적조직표체모식，결과발현，CAM5기인재의남개식주적근、경、협、화급유묘중균유표체。차외，감정료의남개CAM5기인적순합돌변체 cam5，병관찰기협병외식체재유상조직유도과정중적표형，결과발현，재유상유도배양기중배양20 d，여야생형상비，돌변체적협병외식체팽대비교명현。진일보분석현시，배양20 d 후，돌변체외식체적선중비배양전증가료39배，이야생형부증가료16배。저사결과암시CAM5기인삼여료유상조직형성。
Callus formation is an important stage during plant tissue culture,in which the content of calmodulin changes.Howev-er,whether calmodulins participate in callus formation still remains unknown.Therefore,in the paper,the tissue expression pat-tern of a calmodulin gene of CAM5 was analyzed.The results show that CAM5 gene is expressed in Arabidopsis roots,stems, leaves,inflorescence and seedlings.In addition,the cam5 homozygous mutant of Arabidopsis CAM5 gene is identified and the phenotype of cam5 petiole explants is observed in the course of callus induction.The results indicate that the callus on the cam5 petiole explants is dilated more obviously than those on wild-type explants after twenty-day culture on callus inducing medium. Further analysis indicates that the fresh weight of cam5 explants increases 39 times after twenty-day culture than before culture, while the wild type increased only 16 times.These results suggest that CAM5 gene is involved in callus formation.