CAJ | 학술논문

目的：探讨超声心动图在评价酒精性心肌病大鼠心脏结构和功能中的应用价值。方法选取雄性Wistar大鼠45只，随机分为模型2月组（10只）、模型4月组（12只）、模型6月组（13只）及正常组（10只）。模型组进行酒精灌胃及酒精随意饮用；正常对照组选择相同时间及相同剂量生理盐水灌胃。分别于2个月、4个月、6个月后进行超声心动图检测，并将模型组与正常组超声结果进行对比。检测结束后处死模型组和正常组大鼠，取心脏组织进行病理检测，将超声结果与病理结果进行对比。结果经超声检查，模型2月组与正常组大鼠心脏超声比较未见明显差异。模型4月组与正常组相比，舒张末期左心室后壁厚度（LVPWDd）、舒张末期室间隔厚度（IVSDd）、左室舒张末期内径（LVEDd）、左室质量（LVM）、LVM与体质量之比（LVM/BM）增大，舒张早期心肌运动峰值速度（Em）、Em/Am减低，且均有显著性差异（P＜0.05）。模型6月组与正常组相比，LVPWDd、IVSDd减小，LVM、LVM/BM、LVEDd增大，射血分数（EF）、左室短轴缩短率（FS）明显减低，Em、Em/Am减低，且均有显著性差异（P＜0.05）。超声测定的LVM与尸检检测结果高度相关（r=0.84，P＜0.05）。经病理检查，模型2月组以及正常组大鼠心肌HE染色未见异常，模型4月组及6月组大鼠心肌HE染色分别符合亚临床期和临床期酒精性心肌病的改变。结论超声心动图可对酒精性心肌病大鼠的心脏结构和功能进行动态、定量的评价。
목적：탐토초성심동도재평개주정성심기병대서심장결구화공능중적응용개치。방법선취웅성Wistar대서45지，수궤분위모형2월조（10지）、모형4월조（12지）、모형6월조（13지）급정상조（10지）。모형조진행주정관위급주정수의음용；정상대조조선택상동시간급상동제량생리염수관위。분별우2개월、4개월、6개월후진행초성심동도검측，병장모형조여정상조초성결과진행대비。검측결속후처사모형조화정상조대서，취심장조직진행병리검측，장초성결과여병리결과진행대비。결과경초성검사，모형2월조여정상조대서심장초성비교미견명현차이。모형4월조여정상조상비，서장말기좌심실후벽후도（LVPWDd）、서장말기실간격후도（IVSDd）、좌실서장말기내경（LVEDd）、좌실질량（LVM）、LVM여체질량지비（LVM/BM）증대，서장조기심기운동봉치속도（Em）、Em/Am감저，차균유현저성차이（P＜0.05）。모형6월조여정상조상비，LVPWDd、IVSDd감소，LVM、LVM/BM、LVEDd증대，사혈분수（EF）、좌실단축축단솔（FS）명현감저，Em、Em/Am감저，차균유현저성차이（P＜0.05）。초성측정적LVM여시검검측결과고도상관（r=0.84，P＜0.05）。경병리검사，모형2월조이급정상조대서심기HE염색미견이상，모형4월조급6월조대서심기HE염색분별부합아림상기화림상기주정성심기병적개변。결론초성심동도가대주정성심기병대서적심장결구화공능진행동태、정량적평개。
ObjectiveToexploretheapplicationvalueofechocardiographyinevaluatingcardiac structureandfunctionofratswithalcoholiccardiomyopathy (ACM). Methods45maleWistarrats wererandomlydividedintonormalgroup (n=10)andACMgroup (n=35).ACMgroupweredivided into 2-monthgroup (n=10), 4-monthgroup (n=12)and 6-monthgroup (n=13).RatsfromACMgroup were gavaged with alcohol, and then let them drink alcohol at will, while rats from normal group were gavagedwithsalineatthesametimewiththesamedose.Thenratsfrom 2-monthgroup, 4-monthgroup and 6-monthgroupwereexaminedbyechocardiography,andtheresultswerecomparedwiththatofnormal group. All the rats were killed after examination. Heart tissues of those dead rats were took out for pathological examination,andtheresultsofultrasoundexaminationandpathologicalexaminationwerecompared. ResuIts Therewasnosignificantdifferencebetweenratsfrom 2-monthgroupandnormalgroup.Compared withthenormalgroup,LVPWDd,IVSDd,LVEDd,LVMandLVM/BMofratsfrom 4-monthgroup increased,whileEmandEm/Amdecreased,thedifferenceswereallsignificant (P<0.05).Compared withthenormalgroup,LVPWDd,IVSDd,EF,FS,Em,andEm/Amofratsfrom 6-monthgroup decreased, while LVM, LVM/BM and LVEDd increased, which were of significant difference (P<0.05).TheLVMmeasuredbyultrasoundwascloselyrelatedtoautopsyresults (r=0.84,P<0.05). Accordingtopathologicalresults,theHEstainingin 2-monthACMratsandnormalratswere normal,whilethatof 4-monthACMratsand 6-monthACMratswererespectivelyinaccordance withthesubclinicalandclinicalperiodofACM. ConcIusionEchocardiographycanbeusedinthe quantitative and dynamical evaluation of the cardiac structure and function of rats with ACM.