CAJ | 학술논문

目的：探讨RNA干扰核因子E2相关因子2（ Nrf2）对食管癌细胞生物学行为的影响。方法建立RNA干扰下调Nrf2表达的Eca?109食管癌细胞（ Si?Nrf2），并构建阴性对照组细胞（ Si?control）。 MTT法、平板克隆形成实验、流式细胞术、Transwell小室实验及Western blotting法检测下调Nrf2表达对食管癌细胞增殖、凋亡及侵袭转移、细胞放射敏感性及相关蛋白表达的影响。结果转染48h 后，Si?Nrf2细胞中Nrf2蛋白相对表达量为0?209±0?013，Si?control细胞中则为0?852±0?077，两者差异具有统计学意义（ P＜0?05）。与Si?control细胞比较，下调Nrf2表达水平后的Si?Nrf2细胞的增殖能力明显减弱、细胞凋亡增多、侵袭转移能力降低，放疗敏感性增高，血红素加氧酶（ HO?1）、抗凋亡蛋白Bcl?2、基质金属蛋白酶?9（ MMP?9）蛋白的表达量明显下调（ P＜0?05）。结论 Nrf2可能通过调控HO?1、Bcl?2及MMP?9蛋白表达参与食管癌细胞增殖、凋亡、侵袭转移及放疗敏感性这一生物学过程。
목적：탐토RNA간우핵인자E2상관인자2（ Nrf2）대식관암세포생물학행위적영향。방법건립RNA간우하조Nrf2표체적Eca?109식관암세포（ Si?Nrf2），병구건음성대조조세포（ Si?control）。 MTT법、평판극륭형성실험、류식세포술、Transwell소실실험급Western blotting법검측하조Nrf2표체대식관암세포증식、조망급침습전이、세포방사민감성급상관단백표체적영향。결과전염48h 후，Si?Nrf2세포중Nrf2단백상대표체량위0?209±0?013，Si?control세포중칙위0?852±0?077，량자차이구유통계학의의（ P＜0?05）。여Si?control세포비교，하조Nrf2표체수평후적Si?Nrf2세포적증식능력명현감약、세포조망증다、침습전이능력강저，방료민감성증고，혈홍소가양매（ HO?1）、항조망단백Bcl?2、기질금속단백매?9（ MMP?9）단백적표체량명현하조（ P＜0?05）。결론 Nrf2가능통과조공HO?1、Bcl?2급MMP?9단백표체삼여식관암세포증식、조망、침습전이급방료민감성저일생물학과정。
Objective To investigate the effect of down?regulation of nuclear factor erythroid 2?related factor( Nrf2) by RNAi on biological behaviors in esophageal cancer cells. Methods Eca?109 cell line which was down?regulated Nrf2 by RNAi( Si?Nrf2) and negative control cell( Si?control) were established. Si?Nrf2 cells and Si?control cells were transfected by Lipofectamine 2000. Cell prolif?eration, apoptosis, cell metastasis and relative protein expression were analyzed by MTT assay, colony formation assay, flow cytometry, transwell and western blotting method after transfection. Results Western blotting showed that the expression of Nrf2 in the Si?Nrf2 cells was 0?209±0?013, which was significantly lower than that in the Si?control cells(0?852±0?077, P<0?05). The result of biologi?cal function shown that Eca?109 cell down?regulated Nrf2 had a lower survival fraction, higher cell apoptosis, and significant decrease in migration and invasion, higher radiosensitivity and lower expression of HO?1, Bcl?2, MMP?9 protein compared with those of Si?con?trol cells( P<0?05) . Conclusion Nrf2 may involve biological process of cell proliferation, apoptosis, invasion and metastasis and ra?diation sensitivity in esophageal cancer by regulating the expression of HO?1, Bcl?2 and MMP?9 protein.