临床肿瘤学杂志
臨床腫瘤學雜誌
림상종류학잡지
CHINESE CLINICAL ONCOLOGY
2014年
12期
1075-1080
,共6页
非小细胞肺癌%微小RNA%miR-223%miR-93%miR-218%诊断
非小細胞肺癌%微小RNA%miR-223%miR-93%miR-218%診斷
비소세포폐암%미소RNA%miR-223%miR-93%miR-218%진단
Non-small cell lung cancer( NSCLC)%microRNA%miR-223%miR-93%miR-218%Diagnosis
目的：探讨非小细胞肺癌（ NSCLC）患者血浆miR?223、miR?93和miR?218水平，分析三者与NSCLC患者临床病理学特征的关系。方法收集本院收治85例NSCLC患者未经治疗前的血浆标本（ NSCLC组），采用实时定量RT?PCR （ qRT?PCR）法检测血浆中miR?223、miR?93和miR?218水平，收集NSCLC患者的临床病理学参数，比较三者不同水平的临床病理参数并分析三者间的关系，采用受试者工作特征曲线（ ROC）评价血浆miR?223、miR?93和miR?218水平检测在NSCLC诊断中的临床价值。同时选取同期的90例健康体检者的血浆标本作对照（对照组）。结果 NSCLC组的血浆miR?223和miR?93水平高于对照组，miR?218水平低于对照组，差异均有统计学意义（ P均＜0?05）；miR?223水平与TNM分期、肿瘤大小有关， miR?93水平与组织学类型、淋巴结转移有关，miR?218水平与肿瘤大小、分化程度有关，以上差异均有统计学意义（ P＜0?05）；NSCLC患者血浆中miR?223水平与miR?93呈正相关（r＝0?411），miR?223、miR?93分别与miR?218呈负相关（r＝－0?361，r＝－0?451），差异均有统计学意义（P＜0?05）；血浆 miR?223、miR?93和 miR?218诊断 NSCLC 的 AUC、灵敏度和特异度分别为0?926、95?2％和87?1％，0?912、88?4％和92?5％，0?941、92?7％和84?4％，均高于CEA的0?774、75?1％和66?2％，且miR?223、miR?93和miR?218联合诊断的效能高于单独检测。结论 NSCLC患者血浆中miR?223和miR?93呈高表达，miR?218呈低表达，与临床病理学参数有关，且在NSCLC诊断中有一定的价值，可用于辅助NSCLC的诊断和病情评估。
目的：探討非小細胞肺癌（ NSCLC）患者血漿miR?223、miR?93和miR?218水平，分析三者與NSCLC患者臨床病理學特徵的關繫。方法收集本院收治85例NSCLC患者未經治療前的血漿標本（ NSCLC組），採用實時定量RT?PCR （ qRT?PCR）法檢測血漿中miR?223、miR?93和miR?218水平，收集NSCLC患者的臨床病理學參數，比較三者不同水平的臨床病理參數併分析三者間的關繫，採用受試者工作特徵麯線（ ROC）評價血漿miR?223、miR?93和miR?218水平檢測在NSCLC診斷中的臨床價值。同時選取同期的90例健康體檢者的血漿標本作對照（對照組）。結果 NSCLC組的血漿miR?223和miR?93水平高于對照組，miR?218水平低于對照組，差異均有統計學意義（ P均＜0?05）；miR?223水平與TNM分期、腫瘤大小有關， miR?93水平與組織學類型、淋巴結轉移有關，miR?218水平與腫瘤大小、分化程度有關，以上差異均有統計學意義（ P＜0?05）；NSCLC患者血漿中miR?223水平與miR?93呈正相關（r＝0?411），miR?223、miR?93分彆與miR?218呈負相關（r＝－0?361，r＝－0?451），差異均有統計學意義（P＜0?05）；血漿 miR?223、miR?93和 miR?218診斷 NSCLC 的 AUC、靈敏度和特異度分彆為0?926、95?2％和87?1％，0?912、88?4％和92?5％，0?941、92?7％和84?4％，均高于CEA的0?774、75?1％和66?2％，且miR?223、miR?93和miR?218聯閤診斷的效能高于單獨檢測。結論 NSCLC患者血漿中miR?223和miR?93呈高錶達，miR?218呈低錶達，與臨床病理學參數有關，且在NSCLC診斷中有一定的價值，可用于輔助NSCLC的診斷和病情評估。
목적：탐토비소세포폐암（ NSCLC）환자혈장miR?223、miR?93화miR?218수평，분석삼자여NSCLC환자림상병이학특정적관계。방법수집본원수치85례NSCLC환자미경치료전적혈장표본（ NSCLC조），채용실시정량RT?PCR （ qRT?PCR）법검측혈장중miR?223、miR?93화miR?218수평，수집NSCLC환자적림상병이학삼수，비교삼자불동수평적림상병리삼수병분석삼자간적관계，채용수시자공작특정곡선（ ROC）평개혈장miR?223、miR?93화miR?218수평검측재NSCLC진단중적림상개치。동시선취동기적90례건강체검자적혈장표본작대조（대조조）。결과 NSCLC조적혈장miR?223화miR?93수평고우대조조，miR?218수평저우대조조，차이균유통계학의의（ P균＜0?05）；miR?223수평여TNM분기、종류대소유관， miR?93수평여조직학류형、림파결전이유관，miR?218수평여종류대소、분화정도유관，이상차이균유통계학의의（ P＜0?05）；NSCLC환자혈장중miR?223수평여miR?93정정상관（r＝0?411），miR?223、miR?93분별여miR?218정부상관（r＝－0?361，r＝－0?451），차이균유통계학의의（P＜0?05）；혈장 miR?223、miR?93화 miR?218진단 NSCLC 적 AUC、령민도화특이도분별위0?926、95?2％화87?1％，0?912、88?4％화92?5％，0?941、92?7％화84?4％，균고우CEA적0?774、75?1％화66?2％，차miR?223、miR?93화miR?218연합진단적효능고우단독검측。결론 NSCLC환자혈장중miR?223화miR?93정고표체，miR?218정저표체，여림상병이학삼수유관，차재NSCLC진단중유일정적개치，가용우보조NSCLC적진단화병정평고。
Objective To explore the levels of plasma miR?223, miR?93 and miR?218 in non?small lung cancer( NSCLC) and analysis the relationship between the 3 indicators and clinicopathological parameters of NSCLC. Methods Plasma samples from 85 NSCLC patients before treatment were collected as NSCLC group. The real?time quantitative RT?PCR( qRT?PCR) was used to detect the levels of miR?223, miR?93 and miR?218 and the clinicopathological parameters of NSCLC patients were collected. The clinicopatho?logical parameters of patients with different levels of miR?223, miR?93 and miR?218 were compared. The receiver operating characteris?tic curve( ROC) was employed to analysis the clinical value of plasma miR?223, miR?93 and miR?218 in the diagnosis of NSCLC. Meanwhile, the plasma samples from 90 healthy volunteers at the same time were selected as control( control group) . Results There were higher levels of miR?223 and miR?93 but lower miR?218 in NSCLC group compaired with control group with significant difference ( P<0?05) . The level of miR?223 was related with TNM stage and tumor size, and miR?93 was related with histological type and lymph node metastasis, and miR?218 was related with tumor size and degree of differentiation, showing with significant difference ( P all<0?05) . The plasma level of miR?223 was positively correlated with miR?93( r=0?411) , but miR?223 and miR?93 were negatively cor?related with miR?218(r=-0?361 and r=-0?451)with statistically significance(P<0?05). The AUC, sensitivity and specificity of plasma miR?223, miR?93 and miR?218 in the diagnosis of NSCLC were 0?926, 95?2% and 87?1%, 0?912, 88?4% and 92?5%, and 0?941, 92?7% and 84?4%, higher than 0?774, 75?1% and 66?2% of carcinoembryonic antigen( CEA) . The combined efficiency of miR?223, miR?93 and miR?218 in NSCLC was superior to that alone. Conclusion There are higher expression of miR?223 and miR?93 but lower expression of miR?218 in NSCLC. The plasma levels of the above indicators are related with clinical pathology parameters, showing a certain value in the diagnosis of NSCLC as assisted indicators in the diagnosis of NSCLC.
