CAJ | 학술논문

目的：探讨磷酸酰肌醇3激酶/蛋白激酶B( phosphoinositide 3 kinese/protein kinase B, PI3K/Akt)信号通路抑制因子PTEN与糖尿病肾病( diabetic nephropathy, DN)患者足细胞损伤的关系。方法收集30例DN患者和10例健康志愿者的24 h尿标本,采用酶联免疫吸附实验( enzyme linked immunosorbent assay, ELISA)检测患者尿液中足盂蛋白( podocalyxin, PCX)的水平；肾活检组织进行形态学观察,根据肾小球病变将DN患者分为3组,免疫组化法检测各组肾小球内p-Akt和PTEN的表达。结果 DN患者尿液中PCX的水平明显高于健康对照组,并随肾小球病变加重其水平逐渐上升；p-Akt和PTEN在DN患者肾小球的表达有所上调,但随着肾小球病变的加重其表达逐渐减少；DN患者尿液中PCX水平与PTEN表达呈负相关,与24 h尿蛋白呈正相关。结论 PTEN表达下调可能通过改变Akt的活化状态,从而在DN患者足细胞损伤中发挥一定作用。
목적：탐토린산선기순3격매/단백격매B( phosphoinositide 3 kinese/protein kinase B, PI3K/Akt)신호통로억제인자PTEN여당뇨병신병( diabetic nephropathy, DN)환자족세포손상적관계。방법수집30례DN환자화10례건강지원자적24 h뇨표본,채용매련면역흡부실험( enzyme linked immunosorbent assay, ELISA)검측환자뇨액중족우단백( podocalyxin, PCX)적수평；신활검조직진행형태학관찰,근거신소구병변장DN환자분위3조,면역조화법검측각조신소구내p-Akt화PTEN적표체。결과 DN환자뇨액중PCX적수평명현고우건강대조조,병수신소구병변가중기수평축점상승；p-Akt화PTEN재DN환자신소구적표체유소상조,단수착신소구병변적가중기표체축점감소；DN환자뇨액중PCX수평여PTEN표체정부상관,여24 h뇨단백정정상관。결론 PTEN표체하조가능통과개변Akt적활화상태,종이재DN환자족세포손상중발휘일정작용。
Purpose To investigate the role of PTEN in podocyte injury in patients with diabetic nephropathy ( DN) . Methods Uri-nary samples from 30 patients with DN and 10 healthy volunteers were collected to detect the level of PCX by ELISA. Renal biopsies were reviewed to observe the morphological changes. All patients with DN were divided into three groups by glomerular lesion. The ex-pression of p-Akt and PTEN in glomeruli was detected by immunohistochemistry. Results The levels of PCX in the urine were signifi-cantly higher in patients with DN compared with those in healthy volunteers, and gradually increased along with glomerular lesion aggra-vating. The expression of p-Akt and PTEN increased in patients with DN compared with healthy volunteers. Although the expression of p-Akt and PTEN decreased with the aggravation of glomerular lesion, they were still higher than that in volunteers. There were obvious-ly positive correlation between the level of PCX and 24-h urinary protein and negative correlation between the level of PCX and the ex-pression level of p-Akt and PTEN. Conclusion PTEN down-regulation may be associated with podocyte injury in DN, which may be associated with the phosphorylation of Akt.