CAJ | 학술논문

目的：探索糖尿病大鼠肾脏拼接型X盒子结合蛋白1(spliced X-box binding protein-1, XBP-1s)和脂肪分化相关蛋白( adipose differentiation related protein, ADRP)在糖尿病大鼠肾脏组织中的表达及二者表达的相关性。方法腹腔注射STZ成功构建糖尿病大鼠模型,正常喂养2个月后,采用免疫组化和Western blot技术检测大鼠肾脏组织中XBP-1s和ADRP的表达。结果 XBP-1s和ADRP表达均定位于肾小管上皮细胞,对照大鼠相比,糖尿病大鼠肾小管上皮细胞中XBP-1s和ADRP表达均显著增高,分别升高2.017倍和1.544倍,差异有统计学意义(t=2．648、2．741,P<0．05)。进一步行相关性分析显示：大鼠肾脏组织中XBP-1s表达增强往往伴随着ADRP的表达增强(相关系数为0．723,P<0．05)。结论 XBP-1s表达升高可能是导致糖尿病大鼠肾脏组织中ADRP上调的因素之一。
목적：탐색당뇨병대서신장병접형X합자결합단백1(spliced X-box binding protein-1, XBP-1s)화지방분화상관단백( adipose differentiation related protein, ADRP)재당뇨병대서신장조직중적표체급이자표체적상관성。방법복강주사STZ성공구건당뇨병대서모형,정상위양2개월후,채용면역조화화Western blot기술검측대서신장조직중XBP-1s화ADRP적표체。결과 XBP-1s화ADRP표체균정위우신소관상피세포,대조대서상비,당뇨병대서신소관상피세포중XBP-1s화ADRP표체균현저증고,분별승고2.017배화1.544배,차이유통계학의의(t=2．648、2．741,P<0．05)。진일보행상관성분석현시：대서신장조직중XBP-1s표체증강왕왕반수착ADRP적표체증강(상관계수위0．723,P<0．05)。결론 XBP-1s표체승고가능시도치당뇨병대서신장조직중ADRP상조적인소지일。
Purpose To investigate the expression of XBP-1s and ADRP in kidney of diabetic rats. Methods Diabetic rat models were successfully established by intraperitoneal injection of STZ. After two months rats were sacrificed and XBP-1s and ADRP were de-tected by immunohistochemistry and Western blot. Results XBP-1s and ADRP were located in renal tubular cells and increased by a-bout 2. 017 times and 1. 544 times in comparison with normal control rats (P<0. 05). Moreover, it was shown that high expression of XBP-1s was commonly accompanied with increased ADRP by Pearson correlation analysis and the correlation coefficient was 0. 723 (P<0. 05). Conclusion The increased XBP-1s may cause the up-regulation of ADRP in the kidney of diabetic rats.