CAJ | 학술논문

目的：通过N-甲基-N’-硝基-N-亚硝基胍(MNNG)处理大鼠肝卵圆细胞系WB-F344,使其发生恶性转化,为研究肝癌的发生、发展提供有用的体外模型。方法以MNNG作用于WB-F344细胞系,使之发生恶性转化,并从形态学、遗传学、软琼脂克隆形成、NOD/SCID小鼠成瘤等方面加以验证。结果经MNNG诱导数代的WB-F344细胞出现恶性特征,表现为细胞形态改变,异倍体核型增多,肿瘤标志物表达增高,非锚定克隆能力增强,接种于NOD/SCID小鼠皮下可成瘤。结论肝卵圆细胞系WB-F344在MNNG的作用下可成为肝癌发生的有效体外模型。
목적：통과N-갑기-N’-초기-N-아초기고(MNNG)처리대서간란원세포계WB-F344,사기발생악성전화,위연구간암적발생、발전제공유용적체외모형。방법이MNNG작용우WB-F344세포계,사지발생악성전화,병종형태학、유전학、연경지극륭형성、NOD/SCID소서성류등방면가이험증。결과경MNNG유도수대적WB-F344세포출현악성특정,표현위세포형태개변,이배체핵형증다,종류표지물표체증고,비묘정극륭능력증강,접충우NOD/SCID소서피하가성류。결론간란원세포계WB-F344재MNNG적작용하가성위간암발생적유효체외모형。
Purpose To study the malignant transformation after treating rat oval cell line ( WB-F344 ) with chemical carcinogen N-methyl-N′-nitro-N-nitrosoguanidine ( MNNG) . Methods WB-F344 cells were cultured with MNNG for severe times. The biological characteristics of induced cells were detected through the following methods:to check proliferation activity by flow cytometry analysis, to examine malignant transformation degree of induced cells by soft agar assay and tumor formation in NOD/SCID mice, and to investi-gate the transcriptional and protein levels of hepatocellular carcinoma marker GGT, GST-P by real time-PCR. Results Oval cells in-duced by MNNG showed changes in biological characteristics and malignant molecular markers. Conclusion Hepatic oval cells model is successfully established, which can be confirmed by tumor formation in NOD/SCID mice.