临床与实验病理学杂志
臨床與實驗病理學雜誌
림상여실험병이학잡지
CHINESE JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
2014年
12期
1333-1338
,共6页
吴淑华%胡金龙%何双%温菲菲%孙晨博%田东
吳淑華%鬍金龍%何雙%溫菲菲%孫晨博%田東
오숙화%호금룡%하쌍%온비비%손신박%전동
大肠肿瘤%Beclin1%LC3%mTOR%预后
大腸腫瘤%Beclin1%LC3%mTOR%預後
대장종류%Beclin1%LC3%mTOR%예후
colorectal neoplasms%Beclin1%LC3%mTOR%prognosis
目的：探讨Beclin1、LC3和mTOR在大肠癌中的表达及意义。方法采用免疫组化EnVision法、Western blot和RT-PCR技术检测Beclin1、LC3和mTOR在大肠癌中的表达,并结合临床病理因素进行分析。结果 Beclin1、LC3和mTOR在大肠癌中的阳性率分别为90．50%、87．19%和46．28%,均明显高于癌旁组织( P<0．05),且LC3蛋白在中+低分化大肠癌中的表达(91．34%、89．58%)高于高分化大肠癌(77．61%),在有淋巴结转移组的表达(95．41%)高于无淋巴结转移组(80．45%), mTOR在有淋巴结转移组的表达(57．80%)高于无淋巴结转移组(35．33%)(P<0．05),Beclin1过表达与大肠癌分化程度和淋巴结转移均无关(P>0．05)。 LC3与Beclin1在大肠癌中表达呈正相关(rs =0．593,P<0．01),与mTOR表达呈负相关(rs =-0．165,P<0．01),Beclin1和mTOR表达之间无明显相关性(P>0．05)。 Kaplan-Meier生存分析显示,Beclin1和LC3均阳性、mTOR阴性、无淋巴结转移的大肠癌患者5年生存率高于Beclin1和LC3均阴性、mTOR阳性及有淋巴结转移的患者。多因素分析显示,LC3、mTOR和淋巴结转移是影响大肠癌预后的独立因素。 RT-PCR和Western blot检测结果显示：Beclin1、LC3和mTOR mRNA和蛋白表达水平均明显高于癌旁组织(P<0．05)。结论自噬相关基因Beclin1、LC3和mTOR的异常表达可能与大肠癌的发生、发展及预后相关；联合检测Beclin1、LC3和mTOR在大肠癌中的表达有助于评估进展程度和预后判断。
目的：探討Beclin1、LC3和mTOR在大腸癌中的錶達及意義。方法採用免疫組化EnVision法、Western blot和RT-PCR技術檢測Beclin1、LC3和mTOR在大腸癌中的錶達,併結閤臨床病理因素進行分析。結果 Beclin1、LC3和mTOR在大腸癌中的暘性率分彆為90．50%、87．19%和46．28%,均明顯高于癌徬組織( P<0．05),且LC3蛋白在中+低分化大腸癌中的錶達(91．34%、89．58%)高于高分化大腸癌(77．61%),在有淋巴結轉移組的錶達(95．41%)高于無淋巴結轉移組(80．45%), mTOR在有淋巴結轉移組的錶達(57．80%)高于無淋巴結轉移組(35．33%)(P<0．05),Beclin1過錶達與大腸癌分化程度和淋巴結轉移均無關(P>0．05)。 LC3與Beclin1在大腸癌中錶達呈正相關(rs =0．593,P<0．01),與mTOR錶達呈負相關(rs =-0．165,P<0．01),Beclin1和mTOR錶達之間無明顯相關性(P>0．05)。 Kaplan-Meier生存分析顯示,Beclin1和LC3均暘性、mTOR陰性、無淋巴結轉移的大腸癌患者5年生存率高于Beclin1和LC3均陰性、mTOR暘性及有淋巴結轉移的患者。多因素分析顯示,LC3、mTOR和淋巴結轉移是影響大腸癌預後的獨立因素。 RT-PCR和Western blot檢測結果顯示：Beclin1、LC3和mTOR mRNA和蛋白錶達水平均明顯高于癌徬組織(P<0．05)。結論自噬相關基因Beclin1、LC3和mTOR的異常錶達可能與大腸癌的髮生、髮展及預後相關；聯閤檢測Beclin1、LC3和mTOR在大腸癌中的錶達有助于評估進展程度和預後判斷。
목적：탐토Beclin1、LC3화mTOR재대장암중적표체급의의。방법채용면역조화EnVision법、Western blot화RT-PCR기술검측Beclin1、LC3화mTOR재대장암중적표체,병결합림상병리인소진행분석。결과 Beclin1、LC3화mTOR재대장암중적양성솔분별위90．50%、87．19%화46．28%,균명현고우암방조직( P<0．05),차LC3단백재중+저분화대장암중적표체(91．34%、89．58%)고우고분화대장암(77．61%),재유림파결전이조적표체(95．41%)고우무림파결전이조(80．45%), mTOR재유림파결전이조적표체(57．80%)고우무림파결전이조(35．33%)(P<0．05),Beclin1과표체여대장암분화정도화림파결전이균무관(P>0．05)。 LC3여Beclin1재대장암중표체정정상관(rs =0．593,P<0．01),여mTOR표체정부상관(rs =-0．165,P<0．01),Beclin1화mTOR표체지간무명현상관성(P>0．05)。 Kaplan-Meier생존분석현시,Beclin1화LC3균양성、mTOR음성、무림파결전이적대장암환자5년생존솔고우Beclin1화LC3균음성、mTOR양성급유림파결전이적환자。다인소분석현시,LC3、mTOR화림파결전이시영향대장암예후적독립인소。 RT-PCR화Western blot검측결과현시：Beclin1、LC3화mTOR mRNA화단백표체수평균명현고우암방조직(P<0．05)。결론자서상관기인Beclin1、LC3화mTOR적이상표체가능여대장암적발생、발전급예후상관；연합검측Beclin1、LC3화mTOR재대장암중적표체유조우평고진전정도화예후판단。
Purpose To investigate the expression of Beclin1, LC3 and mTOR in colorectal cancer ( CRC) and their significance. Methods Immunohistochemistry, Western blot and real-time PCR were employed to detect the expression of Beclin1, LC3 and mTOR in CRC. Results The positive expression rate of Beclin1, LC3 and mTOR in 242 cases of CRC was 90. 50%, 87. 19% and 46. 28%, respectively, which were higher than that in adjacent tissues ( P<0. 05 ) . Moreover, the expression of LC3 in moderately and poorly differentiated CRC was higher than that in well differentiated CRC, and the positive rate of LC3 in CRC with lymph node metastasis was higher than that in CRC without lymph node metastasis. The overexpression of mTOR was related to lymph node metasta-sis (P<0. 05), but both differentiation degree and lymph node metastasis were not associated with Beclin1 (P>0. 05). The expres-sion of LC3 was positively correlated with Beclin1 and negatively correlated with mTOR in colorectal cancer (rs =0. 593, P<0. 01, rs= -0. 165, P<0. 01), and the expression of Beclin1 was not associated with mTOR (P>0. 05). Kaplan-Meier survival analysis re-vealed that the five-year survival rate of patients without nodal metastasis, positive expression of Beclin1, LC3 and negative expression of mTOR was higher than those with nodal metastasis, negative expression of Beclin1 and LC3, and positive expression of mTOR. Cox survival analysis results revealed that LC3, mTOR and lymphnode metastasis were independent prognostic factors. The results of IHC, real-time PCR and Western blot in fresh CRC tissues indicated that the expression of Beclin1, LC3 and mTOR in colorectal cancer was significantly higher than that in adjacent tissues (P<0. 05). Conclusions The aberrant expression of Beclin1, LC3 and mTOR may be associated with the development and progression of colorectal cancer. The simultaneous detection of Beclin1, LC3 and mTOR genes in colorectal cancer may be helpful for the evaluation of the progressive degree and the judgment of prognosis.
