CAJ | 학술논문

目的：探讨鼠神经生长因子(mNGF)对癫痫(EP)幼鼠海马金属硫蛋白Ⅰ/Ⅱ(MTⅠ/Ⅱ)和细胞色素C(Cyt C)表达的影响。方法50只日龄19 d 的SD大鼠，随机分为对照组，EP组，mNGF低、中、高剂量组，每组10只。对照组每日予生理盐水注射；EP组腹腔注射戊四氮40 mg/(kg·d)，连续21 d；mNGF低、中、高剂量组给予等剂量戊四氮21 d后，分别予mNGF 500 AU/(kg·d)、1000 AU/(kg·d)、2000 AU/(kg·d)肌肉注射，连续7 d。观察幼鼠体质量及行为学表现，免疫组织化学法检测海马MTⅠ/Ⅱ和Cyt C阳性细胞表达，实时荧光定量PCR法检测MTⅠ和Cyt C mRNA表达。结果对照组、EP组以及mNGF低、中、高剂量组幼鼠海马的MTⅠ和Cyt C mRNA表达以及MTⅠ/Ⅱ和Cyt C阳性细胞数的差异有统计学意义(F=15.98~105.76，P均=0.000)。两两比较发现，EP组的MTⅠ和Cyt C mRNA表达明显升高，MTⅠ/Ⅱ和Cyt C阳性细胞数明显增多，均分别高于对照组和mNGF低、中、高剂量组，差异有统计学意义(P均<0.05)；低剂量组的MT Ⅰ和Cyt C mRNA表达高于中、高剂量组，而MTⅠ/Ⅱ和Cyt C阳性细胞数高于高剂量组，差异有统计学意义(P均<0.05)；中剂量与高剂量组间MTⅠ和Cyt C mRNA表达以及MTⅠ/Ⅱ和Cyt C阳性细胞数的差异无统计学意义(P均>0.05)。结论 MTⅠ/Ⅱ作为一种应激性蛋白，与Cyt C共同参与了癫痫的过程。mNGF对癫痫后的海马有保护作用，且有剂量依赖。
목적：탐토서신경생장인자(mNGF)대전간(EP)유서해마금속류단백Ⅰ/Ⅱ(MTⅠ/Ⅱ)화세포색소C(Cyt C)표체적영향。방법50지일령19 d 적SD대서，수궤분위대조조，EP조，mNGF저、중、고제량조，매조10지。대조조매일여생리염수주사；EP조복강주사무사담40 mg/(kg·d)，련속21 d；mNGF저、중、고제량조급여등제량무사담21 d후，분별여mNGF 500 AU/(kg·d)、1000 AU/(kg·d)、2000 AU/(kg·d)기육주사，련속7 d。관찰유서체질량급행위학표현，면역조직화학법검측해마MTⅠ/Ⅱ화Cyt C양성세포표체，실시형광정량PCR법검측MTⅠ화Cyt C mRNA표체。결과대조조、EP조이급mNGF저、중、고제량조유서해마적MTⅠ화Cyt C mRNA표체이급MTⅠ/Ⅱ화Cyt C양성세포수적차이유통계학의의(F=15.98~105.76，P균=0.000)。량량비교발현，EP조적MTⅠ화Cyt C mRNA표체명현승고，MTⅠ/Ⅱ화Cyt C양성세포수명현증다，균분별고우대조조화mNGF저、중、고제량조，차이유통계학의의(P균<0.05)；저제량조적MT Ⅰ화Cyt C mRNA표체고우중、고제량조，이MTⅠ/Ⅱ화Cyt C양성세포수고우고제량조，차이유통계학의의(P균<0.05)；중제량여고제량조간MTⅠ화Cyt C mRNA표체이급MTⅠ/Ⅱ화Cyt C양성세포수적차이무통계학의의(P균>0.05)。결론 MTⅠ/Ⅱ작위일충응격성단백，여Cyt C공동삼여료전간적과정。mNGF대전간후적해마유보호작용，차유제량의뢰。
Objective To explore the effects of mouse nerve growth factor (mNGF) on expression of metallothionein I/II (MT I/II) and cytochrome C (Cyt C) in hippocampus of pentylenetetrazol (PTZ)-induced epileptic (EP) young rats. Methods Fif-ty SD rats aged 19 days were randomly divided into control group, EP group, mNGF low, medium, and high dose groups. Each group had 10 rats. Control group was injected with normal saline every day, and EP group was intraperitoneally injected with PTZ 40 mg/(kg·d) for 21 days in succession. The mNGF low, medium, and high dose groups were respectively intramuscularly injected with mNGF 500, 1 000, 2 000 AU/(kg·d) for 7 days in succession after PTZ injection. Changes of body weight, behav-ioral performance were recorded. The positive cells of MT I/II, Cyt C were examined by immunohistochemisty. The levels of MT I, Cyt C mRNA in hippocampus were measured by real-time PCR. Results The number of MT I/II, Cyt C positive cells and the levels of MT I, Cyt C mRNA in hippocampus had signiifcant differences among groups (F=15.98-105.76, P=0.000). The number of MT I/II, Cyt C positive cells and the levels of MT I, Cyt C mRNA of EP group were higher than those in control group, mNGF low, medium, and high dose groups (P<0.05). The number of MT I/II, Cyt C positive cells of mNGF low group were higher than those in mNGF high dose group (P<0.05). The levels of MT I, Cyt C mRNA of mNGF low group were higher than those in mNGF medium and high dose groups (P<0.05). The number of MT I/II, Cyt C positive cells and the levels of MT I, Cyt C mRNA had no differences between mNGF medium and high dose groups (P>0.05). Conclusions As a stress protein, metallothionein is involved in the process of chronic epilepsy along with Cyt C. mNGF has neuroprotective effects on the hippocampus of epileptic rats in dose dependent manner.