临床儿科杂志
臨床兒科雜誌
림상인과잡지
2014年
12期
1150-1154
,共5页
周翠臻%谢利剑%黄敏%王韧健%沈捷%肖婷婷
週翠臻%謝利劍%黃敏%王韌健%瀋捷%肖婷婷
주취진%사리검%황민%왕인건%침첩%초정정
川崎病%基因芯片%丙种球蛋白%基质金属蛋白酶-9
川崎病%基因芯片%丙種毬蛋白%基質金屬蛋白酶-9
천기병%기인심편%병충구단백%기질금속단백매-9
Kawasaki disease%gene chip%immunoglobulin%metalloproteinase 9
目的：探讨静脉注射丙种球蛋白(IVIG)治疗川崎病(KD)的作用机制。方法选择36例KD患儿及13例肺炎和上呼吸道感染患儿(对照组)，应用Agilent基因表达谱芯片检测4例KD患儿(男3例、女1例)在IVIG治疗前后，以及1例3个月男性肺炎患儿外周血白细胞基因mRNA表达谱；其余32例KD和12例对照组患儿应用RT-PCR方法对表达差异基因进行验证分析。结果根据差异基因筛选标准(倍数≥2.0)，发现KD患儿S100A12、S100A9、IL-1β、MMP9等基因IVIG治疗后表达水平比治疗前明显下调。经RT-PCR检测发现，IVIG治疗前组、IVIG治疗后组与对照组三组间IL-1β、S100A12、MMP9 mRNA表达的差异有统计学意义(P均<0.01)。与IVIG治疗前比较，KD患儿IL-1β和MMP9 mRNA表达在治疗后明显下调，差异有统计学意义(P均<0.01)；KD患儿IL-1β mRNA表达水平在IVIG治疗前高于对照组，MMP9 mRNA表达水平在IVIG治疗前、后均高于对照组，差异有统计学意义(P均<0.01)。4例KD合并冠状动脉损害(CAL)患儿及28例无合并CAL患儿在IVIG治疗前、后的MMP9和IL-1β mRNA表达水平差异有统计学意义(P均=0.001)。结论 IL-1β、S100A9、S100A12、MMP9等细胞因子在IVIG治疗KD中发挥了重要作用，其中MMP9基因的表达水平可能与KD合并CAL有关。
目的：探討靜脈註射丙種毬蛋白(IVIG)治療川崎病(KD)的作用機製。方法選擇36例KD患兒及13例肺炎和上呼吸道感染患兒(對照組)，應用Agilent基因錶達譜芯片檢測4例KD患兒(男3例、女1例)在IVIG治療前後，以及1例3箇月男性肺炎患兒外週血白細胞基因mRNA錶達譜；其餘32例KD和12例對照組患兒應用RT-PCR方法對錶達差異基因進行驗證分析。結果根據差異基因篩選標準(倍數≥2.0)，髮現KD患兒S100A12、S100A9、IL-1β、MMP9等基因IVIG治療後錶達水平比治療前明顯下調。經RT-PCR檢測髮現，IVIG治療前組、IVIG治療後組與對照組三組間IL-1β、S100A12、MMP9 mRNA錶達的差異有統計學意義(P均<0.01)。與IVIG治療前比較，KD患兒IL-1β和MMP9 mRNA錶達在治療後明顯下調，差異有統計學意義(P均<0.01)；KD患兒IL-1β mRNA錶達水平在IVIG治療前高于對照組，MMP9 mRNA錶達水平在IVIG治療前、後均高于對照組，差異有統計學意義(P均<0.01)。4例KD閤併冠狀動脈損害(CAL)患兒及28例無閤併CAL患兒在IVIG治療前、後的MMP9和IL-1β mRNA錶達水平差異有統計學意義(P均=0.001)。結論 IL-1β、S100A9、S100A12、MMP9等細胞因子在IVIG治療KD中髮揮瞭重要作用，其中MMP9基因的錶達水平可能與KD閤併CAL有關。
목적：탐토정맥주사병충구단백(IVIG)치료천기병(KD)적작용궤제。방법선택36례KD환인급13례폐염화상호흡도감염환인(대조조)，응용Agilent기인표체보심편검측4례KD환인(남3례、녀1례)재IVIG치료전후，이급1례3개월남성폐염환인외주혈백세포기인mRNA표체보；기여32례KD화12례대조조환인응용RT-PCR방법대표체차이기인진행험증분석。결과근거차이기인사선표준(배수≥2.0)，발현KD환인S100A12、S100A9、IL-1β、MMP9등기인IVIG치료후표체수평비치료전명현하조。경RT-PCR검측발현，IVIG치료전조、IVIG치료후조여대조조삼조간IL-1β、S100A12、MMP9 mRNA표체적차이유통계학의의(P균<0.01)。여IVIG치료전비교，KD환인IL-1β화MMP9 mRNA표체재치료후명현하조，차이유통계학의의(P균<0.01)；KD환인IL-1β mRNA표체수평재IVIG치료전고우대조조，MMP9 mRNA표체수평재IVIG치료전、후균고우대조조，차이유통계학의의(P균<0.01)。4례KD합병관상동맥손해(CAL)환인급28례무합병CAL환인재IVIG치료전、후적MMP9화IL-1β mRNA표체수평차이유통계학의의(P균=0.001)。결론 IL-1β、S100A9、S100A12、MMP9등세포인자재IVIG치료KD중발휘료중요작용，기중MMP9기인적표체수평가능여KD합병CAL유관。
Objective To explore the mechanism of intravenous immunoglobulin (IVIG) treatment on Kawasaki disease (KD). Methods Thirty-six KD patients and 13 patients with pneumonia or upper respiratory infection (control group) were se-lected. The gene expression proifles of peripheral white blood cells from 4 KD patients (three male, one female) pre-and post-IVIG therapy and one pneumonia patient (male) were analyzed by Agilent gene chip. The gene expression was detected in 32 KD patients and 12 control patients by real-time PCR. Results The expressions of IL-1β, S100A9, S100A12 and MMP9 were signiif-cantly down-regulated in response to IVIG. The expressions of IL-1β, S100A9, S100A12 and MMP9 mRNA were signiifcantly different among pre-treatment, post-treatment and control groups (P<0.01). The expressions of IL-1βand MMP9 mRNA were signiifcantly down-regulated in response to IVIG (P<0.01). The expression of IL-1βmRNA was signiifcantly higher in KD pa-tients than that in control group. The expression of MMP9 mRNA was signiifcantly higher in KD patients pre and post treatment than that in control group (P<0.01). The expression of MMP9 mRNA was signiifcantly higher in KD patients complicated with coronary artery lesion (CAL) than that in KD patients without CAL complication (P=0.001). Conclusions The effects of IVIG on KD may be mediated by IL1B, S100A9, S100A12 and MMP9. MMP9 gene expression may be related to complication of CAL in KD.
