CAJ | 학술논문

目的探讨人参皂苷（ GSS）含药血清对成骨细胞（ OB）增殖、分化和矿化能力的影响。方法源于大鼠颅盖骨的原代OB中加入不同浓度的人参皂苷含药血清进行干预。 MTT法观察人参皂苷含药血清对OB增殖的影响，用硝基苯磷酸盐（ PNPP）偶氮法观察人参皂苷含药血清对OB碱性磷酸酶（ ALP）活性的影响，茜素红（ ARS）进行矿化结节染色并计算面积以观察人参皂苷含药血清对OB矿化能力的影响。结果人参皂苷含药血清可使体外培养OB的增殖率明显提高[（2．1990±0．2778）比（1．2165±0．1417），P＜0．01）]，并可明显促进OB的矿化能力[（23．7±5．1） mm2／孔比（9．0±5．1）mm2／孔，P＜0．05]，其对OB的ALP活性影响作用也同样明显[（21．2±3．2） U／mg 比（10．2±2．9）U／mg，P＜0．01]。结论人参皂苷含药血清可促进OB的增殖能力和矿化能力。
목적탐토인삼조감（ GSS）함약혈청대성골세포（ OB）증식、분화화광화능력적영향。방법원우대서로개골적원대OB중가입불동농도적인삼조감함약혈청진행간예。 MTT법관찰인삼조감함약혈청대OB증식적영향，용초기분린산염（ PNPP）우담법관찰인삼조감함약혈청대OB감성린산매（ ALP）활성적영향，천소홍（ ARS）진행광화결절염색병계산면적이관찰인삼조감함약혈청대OB광화능력적영향。결과인삼조감함약혈청가사체외배양OB적증식솔명현제고[（2．1990±0．2778）비（1．2165±0．1417），P＜0．01）]，병가명현촉진OB적광화능력[（23．7±5．1） mm2／공비（9．0±5．1）mm2／공，P＜0．05]，기대OB적ALP활성영향작용야동양명현[（21．2±3．2） U／mg 비（10．2±2．9）U／mg，P＜0．01]。결론인삼조감함약혈청가촉진OB적증식능력화광화능력。
Objective To explore the effects of serum from ginsenoside saponins ( GSS ) on the proliferation, differentiation and mineralization in osteoblasts ( OB ) .Methods Serum from GSS with different doses were fed to calvaria bone specimens from rats.The proliferation of osteoblasts and the alkaline phosphatase ( ALP) activity of OB were analyzed.After alizarin red S staining, the area of calcium nodule was calculated to observe the result of serum from GSS on OB mineralization.Results Serum from GSS had notable effects on the proliferation of in vitro cultured OB ( P <0.01 ) and remarkably stimulated the mineralization of OB (P<0.05) while its effect on the ALP activity of OB was also marked (P<0.01). Conclusion Serum from GSS can promote the proliferation and mineralization ability of OB and significantly accelerate the formation of OB.