CAJ | 학술논문

目的：建立小鼠子宫内膜适度损伤模型。方法采用自制的单极电针插入ICR小鼠一侧子宫腔，以0．5 W功率电凝损伤子宫腔；对侧子宫为对照。比较术后7 d不同侧别单层子宫内膜厚度和相同高倍视野下子宫内膜腺体与血管数目比值。妊娠后17．5 d比较小鼠双侧子宫鼠胎个数差别。结果术后7 d损伤侧小鼠子宫内膜厚度（1．14±0．08）mm，对照侧（1．88±0．15）mm ，两者比较P＜0．05；高倍视野下内膜腺体数目损伤侧（20±2）个，对照侧（32±3）个，两者比较P＜0．05；损伤侧平均鼠胎数目（3±2）只，对侧（8±2）只（下降62．6％），两者比较P＜0．01。结论电凝损伤后小鼠子宫内膜的形态学改变和功能学测试符合子宫损伤模型需要，适用于子宫内膜损伤的动物研究。
목적：건립소서자궁내막괄도손상모형。방법채용자제적단겁전침삽입ICR소서일측자궁강，이0．5 W공솔전응손상자궁강；대측자궁위대조。비교술후7 d불동측별단층자궁내막후도화상동고배시야하자궁내막선체여혈관수목비치。임신후17．5 d비교소서쌍측자궁서태개수차별。결과술후7 d손상측소서자궁내막후도（1．14±0．08）mm，대조측（1．88±0．15）mm ，량자비교P＜0．05；고배시야하내막선체수목손상측（20±2）개，대조측（32±3）개，량자비교P＜0．05；손상측평균서태수목（3±2）지，대측（8±2）지（하강62．6％），량자비교P＜0．01。결론전응손상후소서자궁내막적형태학개변화공능학측시부합자궁손상모형수요，괄용우자궁내막손상적동물연구。
Objective To establish the murine model of moderate endometrial injury.Methods Electrocoagulation was applied to induce endometrial injury of ICR mice with 0.5 watts power while contralateral uterine cavity acted as control without electrocoagulation.The endometrial histomorphology was observed in 7 days later by microscopy and fetal number of each lateral uterus assessed at 17.5 days after pregnancy.Results At 7 days post-electrocoagulation, the average endometrial thickness of operating side was significantly thinner than that of control side (1.14 ±0.08 vs 1.88 ±0.15 mm, P<0.05).The density of endometrial glands of operating side was significantly lower than that of control side (20 ±2 vs 32 ±3 per 100x field, P<0.05).After pregnancy, the average number of embryos at operating side decreased by 63.1% compared with control (3 ±2 vs 8 ±2, P <0.01).Conclusion The established model of endometrial electrocoagulation injury shows morphologic changes and decreased fertile ability.It has potential uses for animal studies of endometrial injury treatment.