CAJ | 학술논문

采用离子液体双水相提取木瓜蛋白酶。首先考察不同浓度、pH、温度的离子液体对木瓜蛋白酶活性的影响，其次考察不同离子液体双水相体系、离子液体侧烷基链长度及浓度、酶添加量、pH、温度对木瓜蛋白酶分配行为的影响。结果表明：[C4mim]Cl 和[C4mim]Br 体系萃取木瓜蛋白酶的效果比[C4mim]BF4体系好；高温（≥60℃）对离子液体双水相体系萃取木瓜蛋白酶不利。离子液体双水相萃取木瓜蛋白酶的最佳工艺条件：0.25 g·ml?1的[C4mim]Cl，0.35 g·ml?1的K2HPO4，pH 8.0，酶添加量2.0 mg·ml?1，30℃。此条件下木瓜蛋白酶的酶活性回收率达到95.16%，纯化因子达到1.5。为今后进一步研究该体系的放大实验或规模化生产奠定了基础。
채용리자액체쌍수상제취목과단백매。수선고찰불동농도、pH、온도적리자액체대목과단백매활성적영향，기차고찰불동리자액체쌍수상체계、리자액체측완기련장도급농도、매첨가량、pH、온도대목과단백매분배행위적영향。결과표명：[C4mim]Cl 화[C4mim]Br 체계췌취목과단백매적효과비[C4mim]BF4체계호；고온（≥60℃）대리자액체쌍수상체계췌취목과단백매불리。리자액체쌍수상췌취목과단백매적최가공예조건：0.25 g·ml?1적[C4mim]Cl，0.35 g·ml?1적K2HPO4，pH 8.0，매첨가량2.0 mg·ml?1，30℃。차조건하목과단백매적매활성회수솔체도95.16%，순화인자체도1.5。위금후진일보연구해체계적방대실험혹규모화생산전정료기출。
Papain was extracted by ionic liquid aqueous two-phase system. Firstly,the influence of concentration, pH, and temperature of ionic liquid on the activity of papain was investigated. Secondly, the effects of different ionic liquid aqueous two-phase systems, alkyl chain lengths and concentrations of the ionic liquid, dosage of papain, pH, temperature on the partitioning behavior of papain were discussed. [C4mim]Cl and [C4mim]Br systems were better than [C4mim]BF4 system in extraction of papain, and it was disadvantageous to extract papain at a high temperature (60℃or higher). Activity recovery of enzyme reached 95.16%and purification factor reached 1.5 under the optimum conditions:[C4mim]Cl 0.25 g·ml?1, K2HPO4 0.35 g·ml?1, pH 8.0, enzyme addition 2.0 mg·ml?1, 30℃. The result laid the experimental basis for further scale-up research and commercial production.