CAJ | 학술논문

以1-乙基-3-甲基咪唑磷酸二乙酯（[Emim]DEP）、1-乙基-3-甲基咪唑醋酸（[Emim]Ac）为研究对象，考察两种离子液体（IL）对酶法降解纤维素过程的影响。采用超声辅助离子液体溶解纤维素，[Emim]DEP和[Emim]Ac分别使纤维素的结晶度降为53.6%和62.3%。在纤维素酶降解再生纤维素的过程中，当IL的加入量小于2.0%时，对酶解过程起促进作用，其中加入量为0.5%时，促进作用最强，酶解率分别提高了11.4%和17.5%。在最优条件下（加入0.5%IL），测量纤维素酶系中各个酶的酶活，结果表明离子液体对β-葡萄糖苷酶起到了明显的促进作用，分别使该酶酶活提高了120%和87%。离子液体降低了纤维二糖对葡聚糖外切酶的抑制作用，提高了酶解效率。
이1-을기-3-갑기미서린산이을지（[Emim]DEP）、1-을기-3-갑기미서작산（[Emim]Ac）위연구대상，고찰량충리자액체（IL）대매법강해섬유소과정적영향。채용초성보조리자액체용해섬유소，[Emim]DEP화[Emim]Ac분별사섬유소적결정도강위53.6%화62.3%。재섬유소매강해재생섬유소적과정중，당IL적가입량소우2.0%시，대매해과정기촉진작용，기중가입량위0.5%시，촉진작용최강，매해솔분별제고료11.4%화17.5%。재최우조건하（가입0.5%IL），측량섬유소매계중각개매적매활，결과표명리자액체대β-포도당감매기도료명현적촉진작용，분별사해매매활제고료120%화87%。리자액체강저료섬유이당대포취당외절매적억제작용，제고료매해효솔。
Taking 1-ethyl-3-methylimidazolium diethyl phosphate ([Emim]DEP) and 1-ethyl-3-methylimidazolium acetate ([Emim]Ac) as research targets, the influence of the two ionic liquids (IL) on the process of enzymatic degradation of cellulose was investigated. By using ultrasound to assist ionic liquids in dissolving cellulose, [Emim]DEP and [Emim]Ac made the crystallinity of cellulose decrease to 53.6%and 62.3%respectively. In the process of enzymatic degradation of regenerated cellulose, when the dosage of IL was less than 2.0%, IL acted as promoter, and when the dosage of IL was 0.5%, the promoting effect of IL was the strongest. Enzymatic hydrolysis rate increased by 11.4% and 17.5% respectively. Under the optimal conditions (add in 0.5% IL), the enzyme activities of every enzyme in the cellulose system were measured. Ionic liquids had an obvious promoting effect on β-glucosidase, increasing enzyme activity by 120% and 87% respectively. Ionic liquids reduced inhibition of exoglucanase by cellubiose, and improved efficiency of enzymatic degradation of cellulose.