中国现代药物应用
中國現代藥物應用
중국현대약물응용
CHINESE JOURNAL OF MODERN DRUG APPLICATION
2015年
2期
219-221
,共3页
李洪泽%齐骁勇%韩风雨%孔新颖%刘福青
李洪澤%齊驍勇%韓風雨%孔新穎%劉福青
리홍택%제효용%한풍우%공신영%류복청
高效液相色谱法%六味首乌颗粒%二苯乙烯苷%含量测定
高效液相色譜法%六味首烏顆粒%二苯乙烯苷%含量測定
고효액상색보법%륙미수오과립%이분을희감%함량측정
High performance liquid chromatography%Liuwei Polygonum multiflorum Thunb granules%Stilbeneglycoside%Content determination
目的：建立高效液相色谱法(HPLC)测定六味首乌颗粒中二苯乙烯苷含量的方法。方法采用高效液相色谱法,色谱柱为Luna 5μm, C18(2),250 mm×4.60 mm 100 A。流动相为甲醇-0.2%磷酸溶液(29∶71),流速为1.0 ml/min,检测波长为320 nm。结果二苯乙烯苷浓度在4.99~29.94μg/ml范围内呈良好的线性关系, r=0.9999；二苯乙烯苷平均回收率为98.42%, RSD为1.04%。结论高效液相色谱法测定方法操作简便、结果准确、重现性好,可用于六味首乌颗粒的质量控制。
目的：建立高效液相色譜法(HPLC)測定六味首烏顆粒中二苯乙烯苷含量的方法。方法採用高效液相色譜法,色譜柱為Luna 5μm, C18(2),250 mm×4.60 mm 100 A。流動相為甲醇-0.2%燐痠溶液(29∶71),流速為1.0 ml/min,檢測波長為320 nm。結果二苯乙烯苷濃度在4.99~29.94μg/ml範圍內呈良好的線性關繫, r=0.9999；二苯乙烯苷平均迴收率為98.42%, RSD為1.04%。結論高效液相色譜法測定方法操作簡便、結果準確、重現性好,可用于六味首烏顆粒的質量控製。
목적：건립고효액상색보법(HPLC)측정륙미수오과립중이분을희감함량적방법。방법채용고효액상색보법,색보주위Luna 5μm, C18(2),250 mm×4.60 mm 100 A。류동상위갑순-0.2%린산용액(29∶71),류속위1.0 ml/min,검측파장위320 nm。결과이분을희감농도재4.99~29.94μg/ml범위내정량호적선성관계, r=0.9999；이분을희감평균회수솔위98.42%, RSD위1.04%。결론고효액상색보법측정방법조작간편、결과준학、중현성호,가용우륙미수오과립적질량공제。
Objective To establish a method for content determination of stilbeneglycoside in Liuwei Polygonum multiflorum Thunb granules by high performance liquid chromatography (HPLC). Methods HPLD was applied by chromatographic column as Luna 5μm, C18(2), 250 mm×4.60 mm 100 A. The mobile phase was methyl alcohol-0.2%phosphoric acid solution (29:71) with 1.0 ml/min of flow rate and 320 nm of detection wavelength. Results The concentration of stilbeneglycoside showed a good linear correlation in the range of 4.99~29.94μg/ml, r=0.9999. The average recovery rate of stilbeneglycoside was 98.42%, RSD=1.04%. Conclusion HPLC has the advantages of easy operation, precise effect, and good reproducibility. It can be applied for quality control of Liuwei Polygonum multiflorum Thunb granules.
