中国现代药物应用
中國現代藥物應用
중국현대약물응용
CHINESE JOURNAL OF MODERN DRUG APPLICATION
2015年
2期
6-7
,共2页
甲状腺微小乳头状癌%冷冻切片%半乳糖凝集素-3%间皮瘤相关抗体-1%细胞角蛋白19%CD56%免疫组化
甲狀腺微小乳頭狀癌%冷凍切片%半乳糖凝集素-3%間皮瘤相關抗體-1%細胞角蛋白19%CD56%免疫組化
갑상선미소유두상암%냉동절편%반유당응집소-3%간피류상관항체-1%세포각단백19%CD56%면역조화
Papillary thyroid microcarcinoma%Freezing microtome section%Galectin-3%Human bone marrow endothelial cell-1%Cytokeratin19%CD56%Immunohistochemistry
目的：探讨结节性甲状腺肿合并甲状腺微小乳头状癌的冷冻切片诊断及半乳糖凝集素-3(Galectin-3)、间皮瘤相关抗体-1(HBME-1)、细胞角蛋白19(CK19)、CD56的表达及意义。方法对40例冷冻切片诊断为甲状腺微小乳头状癌进行光镜观察并采用免疫组化EnVision法检测Galectin-3、HBME-1、CK19、CD56的表达水平。结果40例均为甲状腺微小乳头状癌,光镜下主要组织学特征为毛玻璃样核、核沟、纤维性间质、核内包涵体及砂砾体。免疫组化Galectin-3、HBME-1、CK19在甲状腺微小乳头状癌中均呈阳性表达,在结节性甲状腺肿中呈阴性或个别弱阳性表达。CD56在甲状腺微小乳头状癌6例呈轻度着色,34例呈阴性表达,在周围结节性甲状腺肿组织均呈阳性表达。结论甲状腺微小乳头状癌的诊断主要依据组织病理学, Galectin-3、HBME-1、CK19及CD56的联合检测有助于提高甲状腺微小乳头状癌的诊断准确率。
目的：探討結節性甲狀腺腫閤併甲狀腺微小乳頭狀癌的冷凍切片診斷及半乳糖凝集素-3(Galectin-3)、間皮瘤相關抗體-1(HBME-1)、細胞角蛋白19(CK19)、CD56的錶達及意義。方法對40例冷凍切片診斷為甲狀腺微小乳頭狀癌進行光鏡觀察併採用免疫組化EnVision法檢測Galectin-3、HBME-1、CK19、CD56的錶達水平。結果40例均為甲狀腺微小乳頭狀癌,光鏡下主要組織學特徵為毛玻璃樣覈、覈溝、纖維性間質、覈內包涵體及砂礫體。免疫組化Galectin-3、HBME-1、CK19在甲狀腺微小乳頭狀癌中均呈暘性錶達,在結節性甲狀腺腫中呈陰性或箇彆弱暘性錶達。CD56在甲狀腺微小乳頭狀癌6例呈輕度著色,34例呈陰性錶達,在週圍結節性甲狀腺腫組織均呈暘性錶達。結論甲狀腺微小乳頭狀癌的診斷主要依據組織病理學, Galectin-3、HBME-1、CK19及CD56的聯閤檢測有助于提高甲狀腺微小乳頭狀癌的診斷準確率。
목적：탐토결절성갑상선종합병갑상선미소유두상암적냉동절편진단급반유당응집소-3(Galectin-3)、간피류상관항체-1(HBME-1)、세포각단백19(CK19)、CD56적표체급의의。방법대40례냉동절편진단위갑상선미소유두상암진행광경관찰병채용면역조화EnVision법검측Galectin-3、HBME-1、CK19、CD56적표체수평。결과40례균위갑상선미소유두상암,광경하주요조직학특정위모파리양핵、핵구、섬유성간질、핵내포함체급사력체。면역조화Galectin-3、HBME-1、CK19재갑상선미소유두상암중균정양성표체,재결절성갑상선종중정음성혹개별약양성표체。CD56재갑상선미소유두상암6례정경도착색,34례정음성표체,재주위결절성갑상선종조직균정양성표체。결론갑상선미소유두상암적진단주요의거조직병이학, Galectin-3、HBME-1、CK19급CD56적연합검측유조우제고갑상선미소유두상암적진단준학솔。
Objective To explore the freezing microtome section diagnosis of nodular goiter complicated with papillary thyroid microcarcinoma and the expression and significance of Galectin-3, human bone marrow endothelial cell-1 (HBME-1), cytokeratin19 (CK19) and CD56. Methods A total of 40 diagnosed cases as papillary thyroid microcarcinoma by freezing microtome section were observed by light microscope, and the levels of Galectin-3, HBME-1, CK19 and CD56 were detected by immunohistochemical EnVision method. Results All the cases were papillary thyroid microcarcinoma. Under the light microscope, the main histopathologic features were ground glasslike nuclear, groove, fibrous stromal reactions, nuclear pseudoinclusion and psammoma bodies. The positive staining for Galectin-3, HBME-1 and CK19 was observed in nodular goiter microcarcinoma, but they were negative or mild expression in nodular goiter. There were 6 cases with papillary thyroid microcarcinoma showing mild staining for CD56 and 34 cases revealed negative expression. In the nodular goiter, all cases showed positive straining. Conclusion Diagnosis of papillary thyroid microcarcinoma is mainly based on tissue pathology. The combined detection of Galectin-3, HBME-1, CK19 and CD56 will improve the accuracy of diagnosing papillary thyroid microcarcinoma.
