中国全科医学
中國全科醫學
중국전과의학
CHINESE GENERAL PRACTICE
2015年
3期
294-299
,共6页
唐乾利%黄欣%王宇%何晓微%王兵%黄许森%李辉%金萌%吕震
唐乾利%黃訢%王宇%何曉微%王兵%黃許森%李輝%金萌%呂震
당건리%황흔%왕우%하효미%왕병%황허삼%리휘%금맹%려진
慢性难愈合创面%湿润暴露疗法/ 湿润烧伤膏%病理学%丝裂原活化蛋白激酶激酶%c - myc
慢性難愈閤創麵%濕潤暴露療法/ 濕潤燒傷膏%病理學%絲裂原活化蛋白激酶激酶%c - myc
만성난유합창면%습윤폭로요법/ 습윤소상고%병이학%사렬원활화단백격매격매%c - myc
Chronic Refractory Ulcer%MEBT/ MEBO%Pathology%MAPKK%c - myc
目的:探讨湿润暴露疗法/湿润烧伤膏(MEBT/ MEBO)治疗慢性难愈合创面的超微病理及丝裂原活化蛋白激酶激酶(MAPKK)、c - myc mRNA 表达的机制。方法选取 SPF 级雄性 SD 大鼠80只,采用随机数字表法将其分为空白对照组、模型组、湿润烧伤膏组、康复新液组,每组20只。空白对照组建立全层皮肤缺损开放性创面,模型组、湿润烧伤膏组、康复新液组建立全层皮肤难愈合创面。空白对照组建立创面后不做其他处理,模型组外敷0.9%氯化钠溶液,湿润烧伤膏组外敷 MEBO,康复新液组外喷康复新液。在用药后第3、6、12天利用透射电镜观察大鼠皮肤创面细胞超微结构;用药后第12天进行荧光定量聚合酶链反应(RT - PCR),分析 MAPKK、c - myc mRNA 的表达水平。结果用药后第3、6、12天,湿润烧伤膏组与康复新液组细胞结构逐步改善,各细胞器形态结构逐渐恢复至正常水平。4组 MAPKK、c - myc mRNA 表达水平比较,差异均有统计学意义( P ﹤0.05)。模型组、湿润烧伤膏组与康复新液组较空白对照组 MAPKK、c - myc mRNA 表达水平降低,湿润烧伤膏组、康复新液组较模型组 MAPKK、c - myc mRNA 表达水平均增高(P ﹤0.05);湿润烧伤膏组与康复新液组 MAPKK、c - myc mRNA 表达水平比较,差异无统计学意义(P ﹥0.05)。结论 MEBT/ MEBO 能够改善细胞超微病理结构,提高 MAPKK、c - myc mRNA 的表达水平,促进慢性难愈合创面恢复。
目的:探討濕潤暴露療法/濕潤燒傷膏(MEBT/ MEBO)治療慢性難愈閤創麵的超微病理及絲裂原活化蛋白激酶激酶(MAPKK)、c - myc mRNA 錶達的機製。方法選取 SPF 級雄性 SD 大鼠80隻,採用隨機數字錶法將其分為空白對照組、模型組、濕潤燒傷膏組、康複新液組,每組20隻。空白對照組建立全層皮膚缺損開放性創麵,模型組、濕潤燒傷膏組、康複新液組建立全層皮膚難愈閤創麵。空白對照組建立創麵後不做其他處理,模型組外敷0.9%氯化鈉溶液,濕潤燒傷膏組外敷 MEBO,康複新液組外噴康複新液。在用藥後第3、6、12天利用透射電鏡觀察大鼠皮膚創麵細胞超微結構;用藥後第12天進行熒光定量聚閤酶鏈反應(RT - PCR),分析 MAPKK、c - myc mRNA 的錶達水平。結果用藥後第3、6、12天,濕潤燒傷膏組與康複新液組細胞結構逐步改善,各細胞器形態結構逐漸恢複至正常水平。4組 MAPKK、c - myc mRNA 錶達水平比較,差異均有統計學意義( P ﹤0.05)。模型組、濕潤燒傷膏組與康複新液組較空白對照組 MAPKK、c - myc mRNA 錶達水平降低,濕潤燒傷膏組、康複新液組較模型組 MAPKK、c - myc mRNA 錶達水平均增高(P ﹤0.05);濕潤燒傷膏組與康複新液組 MAPKK、c - myc mRNA 錶達水平比較,差異無統計學意義(P ﹥0.05)。結論 MEBT/ MEBO 能夠改善細胞超微病理結構,提高 MAPKK、c - myc mRNA 的錶達水平,促進慢性難愈閤創麵恢複。
목적:탐토습윤폭로요법/습윤소상고(MEBT/ MEBO)치료만성난유합창면적초미병리급사렬원활화단백격매격매(MAPKK)、c - myc mRNA 표체적궤제。방법선취 SPF 급웅성 SD 대서80지,채용수궤수자표법장기분위공백대조조、모형조、습윤소상고조、강복신액조,매조20지。공백대조조건립전층피부결손개방성창면,모형조、습윤소상고조、강복신액조건립전층피부난유합창면。공백대조조건립창면후불주기타처리,모형조외부0.9%록화납용액,습윤소상고조외부 MEBO,강복신액조외분강복신액。재용약후제3、6、12천이용투사전경관찰대서피부창면세포초미결구;용약후제12천진행형광정량취합매련반응(RT - PCR),분석 MAPKK、c - myc mRNA 적표체수평。결과용약후제3、6、12천,습윤소상고조여강복신액조세포결구축보개선,각세포기형태결구축점회복지정상수평。4조 MAPKK、c - myc mRNA 표체수평비교,차이균유통계학의의( P ﹤0.05)。모형조、습윤소상고조여강복신액조교공백대조조 MAPKK、c - myc mRNA 표체수평강저,습윤소상고조、강복신액조교모형조 MAPKK、c - myc mRNA 표체수평균증고(P ﹤0.05);습윤소상고조여강복신액조 MAPKK、c - myc mRNA 표체수평비교,차이무통계학의의(P ﹥0.05)。결론 MEBT/ MEBO 능구개선세포초미병리결구,제고 MAPKK、c - myc mRNA 적표체수평,촉진만성난유합창면회복。
Objective To study the ultrastructural pathology and the mechanism of expression of MAPKK mRNA & c- myc mRNA of chronic refractory ulcer wound treated by MEBT/ MEBO. Methods A total of 80 SPF male SD rats were randomly divided into four groups by random number table method:blank control group,model group,MEBO ointment group and new healing lotion group,20 rats in each group. The full - thickness skin defect open wound was established among rats in blank control group,full - thickness skin refractory ulcer wound was established among rats in model group,MEBO ointment group and new healing lotion group. After the establishment of wound,rats in blank control group were not managed,rats in model group were dressed with normal saline,rats in MEBO ointment group were dressed with MEBO,rats in new healing lotion group were sprayed with new healing lotion. 3,6,12 days after treatment,the cellular ultrastructural structure of the skin wound was observed using TEM microscope. 12 days after treatment,the expression levels of MAPKK mRNA and c - myc mRNA using RT - PCR technology. Results 3,6 and 12 days after treatment,the cellular ultrastructural structure of the skin wound among rats in MEBO ointment group and new healing lotion group gradually improved,morphology and structure of cell organs restored to normal. There were significant differences in expression levels of MAPKK mRNA and c - myc mRNA among four groups( P ﹤ 0. 05). The expression levels of MAPKK mRNA and c - myc mRNA in model group,MEBO ointment group and new healing lotion group were significantly lower than those in blank control group,respectively;while the expression levels of MAPKK mRNA and c - myc mRNA in MEBO ointment group and new healing lotion group were significantly higher than those in model group,respectively(P ﹤0. 05). There was no significant difference in expression levels of MAPKK mRNA and c - myc mRNA between MEBO ointment group and new healing lotion group( P ﹥ 0. 05). Conclusion MEBT/ MEBO can improve cellular pathological ultrastructure,increase the expression levels of MAPKK mRNA and c - myc mRNA,and promote chronic refractory ulcer wound healing.