中国全科医学
中國全科醫學
중국전과의학
CHINESE GENERAL PRACTICE
2015年
3期
278-282
,共5页
白亚玲%徐金升%张睦清%张胜雷%张俊霞%崔立文%张慧然
白亞玲%徐金升%張睦清%張勝雷%張俊霞%崔立文%張慧然
백아령%서금승%장목청%장성뢰%장준하%최립문%장혜연
肌细胞,平滑肌%维生素 K2%血管钙化%细胞凋亡%大鼠
肌細胞,平滑肌%維生素 K2%血管鈣化%細胞凋亡%大鼠
기세포,평활기%유생소 K2%혈관개화%세포조망%대서
Myocytes,smooth muscle%Vitamin K2%Vascular calcification%Apoptosis%Rats
目的:探讨维生素 K2对高磷诱导的大鼠血管平滑肌细胞(VSMCs)钙化的影响及细胞凋亡在其中所起的作用。方法选取8~10周龄健康雄性 SD 大鼠6只,分别体外分离培养大鼠胸主动脉 VSMCs,采用免疫细胞化学方法鉴定。将 VSMCs 采用随机数字表法分为正常对照组、高磷组、维生素 K2组1(10μmol/ L)、维生素 K2组2(25μmol/ L)及维生素 K2组3(50μmol/ L)。检测 VSMCs 钙含量、Axl mRNA 和 Bcl -2 mRNA 表达、细胞凋亡率。结果5组大鼠 VSMCs 钙含量比较,差异有统计学意义(P ﹤0.05)。高磷组、维生素 K2组1、维生素 K2组2、维生素 K2组3均高于正常对照组(P ﹤0.05);维生素 K2组1、维生素 K2组2、维生素 K2组3均低于高磷组(P ﹤0.05);维生素K2组2、维生素 K2组3均低于维生素 K2组1(P ﹤0.05);维生素 K2组3低于维生素 K2组2(P ﹤0.05)。5组大鼠VSMCs 中 Axl mRNA 表达水平比较,差异有统计学意义(P ﹤0.05)。高磷组、维生素 K2组1 Axl mRNA 表达水平低于正常对照组,维生素 K2组3 Axl mRNA 表达水平高于正常对照组,维生素 K2组2、维生素 K2组3 Axl mRNA 表达水平高于高磷组(P ﹤0.05)。5组大鼠 VSMCs 中 Bcl -2 mRNA 表达水平比较,差异无统计学意义(P ﹥0.05)。5组大鼠细胞凋亡率比较,差异有统计学意义(P ﹤0.05)。高磷组、维生素 K2组1细胞凋亡率高于正常对照组,维生素 K2组1、维生素 K2组2、维生素 K2组3细胞凋亡率低于高磷组(P ﹤0.05)。结论维生素 K2能够抑制高磷诱导的大鼠 VSMCs钙化,其机制可能是通过上调生长停滞特异基因6(Gas6)/ Axl 的表达而抑制了 VSMCs 的凋亡。
目的:探討維生素 K2對高燐誘導的大鼠血管平滑肌細胞(VSMCs)鈣化的影響及細胞凋亡在其中所起的作用。方法選取8~10週齡健康雄性 SD 大鼠6隻,分彆體外分離培養大鼠胸主動脈 VSMCs,採用免疫細胞化學方法鑒定。將 VSMCs 採用隨機數字錶法分為正常對照組、高燐組、維生素 K2組1(10μmol/ L)、維生素 K2組2(25μmol/ L)及維生素 K2組3(50μmol/ L)。檢測 VSMCs 鈣含量、Axl mRNA 和 Bcl -2 mRNA 錶達、細胞凋亡率。結果5組大鼠 VSMCs 鈣含量比較,差異有統計學意義(P ﹤0.05)。高燐組、維生素 K2組1、維生素 K2組2、維生素 K2組3均高于正常對照組(P ﹤0.05);維生素 K2組1、維生素 K2組2、維生素 K2組3均低于高燐組(P ﹤0.05);維生素K2組2、維生素 K2組3均低于維生素 K2組1(P ﹤0.05);維生素 K2組3低于維生素 K2組2(P ﹤0.05)。5組大鼠VSMCs 中 Axl mRNA 錶達水平比較,差異有統計學意義(P ﹤0.05)。高燐組、維生素 K2組1 Axl mRNA 錶達水平低于正常對照組,維生素 K2組3 Axl mRNA 錶達水平高于正常對照組,維生素 K2組2、維生素 K2組3 Axl mRNA 錶達水平高于高燐組(P ﹤0.05)。5組大鼠 VSMCs 中 Bcl -2 mRNA 錶達水平比較,差異無統計學意義(P ﹥0.05)。5組大鼠細胞凋亡率比較,差異有統計學意義(P ﹤0.05)。高燐組、維生素 K2組1細胞凋亡率高于正常對照組,維生素 K2組1、維生素 K2組2、維生素 K2組3細胞凋亡率低于高燐組(P ﹤0.05)。結論維生素 K2能夠抑製高燐誘導的大鼠 VSMCs鈣化,其機製可能是通過上調生長停滯特異基因6(Gas6)/ Axl 的錶達而抑製瞭 VSMCs 的凋亡。
목적:탐토유생소 K2대고린유도적대서혈관평활기세포(VSMCs)개화적영향급세포조망재기중소기적작용。방법선취8~10주령건강웅성 SD 대서6지,분별체외분리배양대서흉주동맥 VSMCs,채용면역세포화학방법감정。장 VSMCs 채용수궤수자표법분위정상대조조、고린조、유생소 K2조1(10μmol/ L)、유생소 K2조2(25μmol/ L)급유생소 K2조3(50μmol/ L)。검측 VSMCs 개함량、Axl mRNA 화 Bcl -2 mRNA 표체、세포조망솔。결과5조대서 VSMCs 개함량비교,차이유통계학의의(P ﹤0.05)。고린조、유생소 K2조1、유생소 K2조2、유생소 K2조3균고우정상대조조(P ﹤0.05);유생소 K2조1、유생소 K2조2、유생소 K2조3균저우고린조(P ﹤0.05);유생소K2조2、유생소 K2조3균저우유생소 K2조1(P ﹤0.05);유생소 K2조3저우유생소 K2조2(P ﹤0.05)。5조대서VSMCs 중 Axl mRNA 표체수평비교,차이유통계학의의(P ﹤0.05)。고린조、유생소 K2조1 Axl mRNA 표체수평저우정상대조조,유생소 K2조3 Axl mRNA 표체수평고우정상대조조,유생소 K2조2、유생소 K2조3 Axl mRNA 표체수평고우고린조(P ﹤0.05)。5조대서 VSMCs 중 Bcl -2 mRNA 표체수평비교,차이무통계학의의(P ﹥0.05)。5조대서세포조망솔비교,차이유통계학의의(P ﹤0.05)。고린조、유생소 K2조1세포조망솔고우정상대조조,유생소 K2조1、유생소 K2조2、유생소 K2조3세포조망솔저우고린조(P ﹤0.05)。결론유생소 K2능구억제고린유도적대서 VSMCs개화,기궤제가능시통과상조생장정체특이기인6(Gas6)/ Axl 적표체이억제료 VSMCs 적조망。
Objective To explore the effects of vitamin K2 on high - phosphorus - induced calcification of rat vascular smooth muscle cells(VSMCs),and the role played by cell apoptosis. Methods A total of 6 healthy female SD rats aged 8 - 10 weeks were selected,VSMCs from rat thoracic aorta were cultured in vitro,and identified by immunocytochemistry. VSMCs were randomly divided into 5 groups by random number table method:control group,high phosphorus group,vitamin K2 group 1(10μmol/ L),vitamin K2 group 2(25 μmol/ L),vitamin K2 group 3(50 μmol/ L). The cell calcium content,the expression of Axl and Bcl - 2 mRNA,and the cell apoptosis rate were measured. Results There was significant difference in calcium content of VSMCs among 5 groups of rats(P ﹤ 0. 05). The VSMCs calcium level in high phosphorus group,vitamin K2 group 1,vitamin K2 group 2,vitamin K2 group 3 was significantly higher than that in control group,respectively( P ﹤ 0. 05). The VSMCs calcium level in vitamin K2 group 1,vitamin K2 group 2,vitamin K2 group 3 was significantly lower than that in high phosphorus group,respectively(P ﹤ 0. 05). The VSMCs calcium level in vitamin K2 group 2,vitamin K2 group 3 was significantly lower than that in vitamin K2 group 1,respectively(P ﹤ 0. 05). The VSMCs calcium level in vitamin K2 group 3 was significantly lower than that in vitamin K2 group 2( P ﹤ 0. 05). There was significant difference in the expression level of Axl mRNA in VSMCs among 5 groups of rats(P ﹤ 0. 05). The expression level of VSMCs Axl mRNA in high phosphorus group and in vitamin K2 group 1 was significantly lower than that in control group,respectively( P ﹤ 0. 05). The expression level of VSMCs Axl mRNA in vitamin K2 group 3 was significantly higher than that in control group(P ﹤ 0. 05). The expression level of VSMCs Axl mRNA in vitamin K2 group 2 and in vitamin K2 group 3 was significantly higher than that in high phosphorus group,respectively (P ﹤ 0. 05). There was no significant difference in the expression level of Bcl - 2 mRNA in VSMCs among 5 groups of rats(P ﹥0. 05). There was significant difference in VSMCs apoptosis rate among 5 groups of rats(P ﹤ 0. 05). The VSMCs apoptosis rate in high phosphorus group and in vitamin K2 group 1 was significantly higher than that in control group,the VSMCs apoptosis rate in vitamin K2 group 1,in vitamin K2 group 2 and in vitamin K2 group 3 was significantly lower than that in high phosphorus group,respectively(P ﹤ 0. 05). Conclusion Vitamin K2 can inhibit high - phosphorus - induced calcification of rat VSMCs, may alleviate VSMCs apoptosis by increasing Gas6 / Axl expression,the mechanism is probably that vitamin K2 may alleviate VSMCs apoptosis by increasing Gas6 / Axl expression.