CAJ | 학술논문

目的：探讨胃癌原发灶和淋巴结转移灶中锌指蛋白139（ ZNF139）与肿瘤细胞凋亡、生存素（Survivin）、B 细胞淋巴瘤/白血病-2（Bcl -2）的关系及意义。方法选取2012年1—6月在河北医科大学第四医院外三科行手术切除的35例进展期胃癌患者为研究对象，收集患者胃癌组织及相应的淋巴结转移组织，采用 DNA 原位末端标记（TUNEL）法检测细胞凋亡指数（AI），反转录-聚合酶链反应（RT - PCR）检测 ZNF139、Survivin、Bcl -2 mRNA 的相对表达水平，蛋白印迹法（Western blotting）检测 ZNF139、Survivin、Bcl -2蛋白表达水平。结果胃癌原发灶 AI（10.8±4.7）高于淋巴结转移灶的（8.7±3.0）（t ＝-2.176，P ＝0.039）。淋巴结转移灶 ZNF139、Survivin、Bcl -2 mRNA 相对表达水平均高于胃癌原发灶（P ﹤0.05）。淋巴结转移灶 ZNF139、Survivin、Bcl -2蛋白表达水平均高于胃癌原发灶（P ﹤0.05）。胃癌原发灶 Survivin mRNA 相对表达水平与 AI 呈负相关（r ＝-0.517，P ＝0.001）；淋巴结转移灶 ZNF139、Survivin、Bcl -2 mRNA 相对表达水平均与 AI 呈负相关（r ＝-0.346、-0.467、-0.511，P ＝0.042、0.005、0.002）。淋巴结转移灶 Bcl -2 mRNA 相对表达水平与胃癌原发灶 Bcl -2 mRNA 相对表达水平呈正相关（r ＝0.791，P ﹤0.001）。在胃癌原发灶中，ZNF139 mRNA 相对表达水平与 Bcl -2 mRNA 相对表达水平呈正相关（r ＝0.482，P ＝0.003）。在淋巴结转移灶中，ZNF139 mRNA 相对表达水平与 Survivin、Bcl -2 mRNA 相对表达水平均呈正相关（r ＝0.436、0.452，P ＝0.009、0.006）；Survivin mRNA 相对表达水平与 Bcl -2 mRNA 相对表达水平呈正相关（r
목적：탐토위암원발조화림파결전이조중자지단백139（ ZNF139）여종류세포조망、생존소（Survivin）、B 세포림파류/백혈병-2（Bcl -2）적관계급의의。방법선취2012년1—6월재하북의과대학제사의원외삼과행수술절제적35례진전기위암환자위연구대상，수집환자위암조직급상응적림파결전이조직，채용 DNA 원위말단표기（TUNEL）법검측세포조망지수（AI），반전록-취합매련반응（RT - PCR）검측 ZNF139、Survivin、Bcl -2 mRNA 적상대표체수평，단백인적법（Western blotting）검측 ZNF139、Survivin、Bcl -2단백표체수평。결과위암원발조 AI（10.8±4.7）고우림파결전이조적（8.7±3.0）（t ＝-2.176，P ＝0.039）。림파결전이조 ZNF139、Survivin、Bcl -2 mRNA 상대표체수평균고우위암원발조（P ﹤0.05）。림파결전이조 ZNF139、Survivin、Bcl -2단백표체수평균고우위암원발조（P ﹤0.05）。위암원발조 Survivin mRNA 상대표체수평여 AI 정부상관（r ＝-0.517，P ＝0.001）；림파결전이조 ZNF139、Survivin、Bcl -2 mRNA 상대표체수평균여 AI 정부상관（r ＝-0.346、-0.467、-0.511，P ＝0.042、0.005、0.002）。림파결전이조 Bcl -2 mRNA 상대표체수평여위암원발조 Bcl -2 mRNA 상대표체수평정정상관（r ＝0.791，P ﹤0.001）。재위암원발조중，ZNF139 mRNA 상대표체수평여 Bcl -2 mRNA 상대표체수평정정상관（r ＝0.482，P ＝0.003）。재림파결전이조중，ZNF139 mRNA 상대표체수평여 Survivin、Bcl -2 mRNA 상대표체수평균정정상관（r ＝0.436、0.452，P ＝0.009、0.006）；Survivin mRNA 상대표체수평여 Bcl -2 mRNA 상대표체수평정정상관（r
Objective To investigate the relationship between ZNF139 and tumor cells apoptosis,Survivin,B - cell lymphoma 2(Bcl - 2)in gastric cancer primary lesions and metastatic lymph nodes and its significance. Methods 35 cases with advanced gastric cancer who underwent surgery in the Third Department of Surgery,the Fourth Hospital of Hebei Medical University from January to June 2012,were selected as study subjects,gastric cancer tissues and the corresponding metastatic lymph nodes were collected. Apoptosis index( AI)was tested with TUNEL assay. The mRNA relative expression levels of ZNF139,Survivin and Bcl - 2 were tested by RT - PCR,the protein expression levels of ZNF139,Survivin and Bcl - 2 were tested with Western blotting assay. Results AI of gastric cancer primary lesions was significantly higher than that in metastatic lymph nodes〔(10. 8 ± 4. 7)vs. (8. 7 ± 3. 0),t = - 2. 176,P = 0. 039〕. The mRNA relative expression levels of ZNF139, Survivin and Bcl - 2 in metastatic lymph nodes were significantly higher than those in gastric cancer primary lesions,respectively (P ﹤ 0. 05). The protein expression levels of ZNF139,Survivin and Bcl - 2 in metastatic lymph nodes were significantly higher than those in gastric cancer primary lesions,respectively( P ﹤ 0. 05). The mRNA relative expression level of Survivin was negatively correlated with AI(r = - 0. 517,P = 0. 001);the mRNA relative expression level of ZNF139,Survivin and Bcl - 2 in metastatic lymph nodes was negatively correlated with AI,respectively( r = - 0. 346, - 0. 467, - 0. 511;P = 0. 042, 0. 005,0. 002). The mRNA relative expression level of Bcl - 2 in metastatic lymph nodes was positively correlated with mRNA relative expression level of Bcl - 2 in gastric cancer primary lesions(r = 0. 791,P ﹤ 0. 001). In gastric cancer primary lesions, the mRNA relative expression level of ZNF139 was positively correlated with mRNA relative expression level of Bcl - 2( r =0. 482,P = 0. 003). In metastatic lymph nodes,the mRNA relative expression level of ZNF139 was positively correlated with mRNA relative expression level of Bcl - 2 and Survivin,respectively(r = 0. 436,0. 452,P = 0. 009,0. 006);mRNA relative expression level of Survivin was positively correlated with mRNA relative expression level of Bcl - 2(r = 0. 465,P = 0. 005). Conclusion The anti - apoptosis ability of gastric cancer cells is strengthened in the process of metastasis,which may be related to the role of ZNF139 in regulating some apoptosis related genes(Survivin,Bcl - 2). However,the relationships of regulation are different between gastric cancer primary lesions and metastatic lymph nodes.