中国实验方剂学杂志
中國實驗方劑學雜誌
중국실험방제학잡지
CHINESE JOURNAL OF EXPERIMENTAL TRADITIONAL MEDICAL FORMULAE
2014年
24期
67-70
,共4页
超高液相色谱%银翘散%绿原酸%连翘苷%牛蒡子苷%橙皮苷%甘草苷
超高液相色譜%銀翹散%綠原痠%連翹苷%牛蒡子苷%橙皮苷%甘草苷
초고액상색보%은교산%록원산%련교감%우방자감%등피감%감초감
UPLC%Yinqiao powder%chlorogenic acid%phillyrin%arctiin%hesperidin%liquiritin
目的:建立UPLC同时测定银翘散中绿原酸、甘草苷、橙皮苷、牛蒡子苷、连翘苷含量的方法.方法:采用UPLC系统,ACQUITY UPLC BEH Shield RP18色谱柱(2.1 mm×50 mm,1.7 μm),甲醇-0.15％甲酸溶液梯度洗脱,流速0.25 mL· min-1,检测波长279 nm,柱温40℃,同时测量5种活性成分的含量.结果:绿原酸、连翘苷、牛蒡子苷、橙皮苷、甘草苷分别在0.018 76 ～0.043 77,0.002 37 ～0.011 85,0.011 21～0.056 06,0.001 34 ～0.006 71,0.001 71 ～0.012 38μg线性关系良好(r≥0.999 1),平均回收率分别为97.12％(RSD 0.9％),101.40％(RSD 2.1％),100.61％(RSD 1.6％),100.79％(RSD 0.7％),101.04％ (RSD 1.7％).结论:该方法简便、快速、专属性强,可用于银翘散的质量控制.
目的:建立UPLC同時測定銀翹散中綠原痠、甘草苷、橙皮苷、牛蒡子苷、連翹苷含量的方法.方法:採用UPLC繫統,ACQUITY UPLC BEH Shield RP18色譜柱(2.1 mm×50 mm,1.7 μm),甲醇-0.15％甲痠溶液梯度洗脫,流速0.25 mL· min-1,檢測波長279 nm,柱溫40℃,同時測量5種活性成分的含量.結果:綠原痠、連翹苷、牛蒡子苷、橙皮苷、甘草苷分彆在0.018 76 ～0.043 77,0.002 37 ～0.011 85,0.011 21～0.056 06,0.001 34 ～0.006 71,0.001 71 ～0.012 38μg線性關繫良好(r≥0.999 1),平均迴收率分彆為97.12％(RSD 0.9％),101.40％(RSD 2.1％),100.61％(RSD 1.6％),100.79％(RSD 0.7％),101.04％ (RSD 1.7％).結論:該方法簡便、快速、專屬性彊,可用于銀翹散的質量控製.
목적:건립UPLC동시측정은교산중록원산、감초감、등피감、우방자감、련교감함량적방법.방법:채용UPLC계통,ACQUITY UPLC BEH Shield RP18색보주(2.1 mm×50 mm,1.7 μm),갑순-0.15％갑산용액제도세탈,류속0.25 mL· min-1,검측파장279 nm,주온40℃,동시측량5충활성성분적함량.결과:록원산、련교감、우방자감、등피감、감초감분별재0.018 76 ～0.043 77,0.002 37 ～0.011 85,0.011 21～0.056 06,0.001 34 ～0.006 71,0.001 71 ～0.012 38μg선성관계량호(r≥0.999 1),평균회수솔분별위97.12％(RSD 0.9％),101.40％(RSD 2.1％),100.61％(RSD 1.6％),100.79％(RSD 0.7％),101.04％ (RSD 1.7％).결론:해방법간편、쾌속、전속성강,가용우은교산적질량공제.
