CAJ | 학술논문

目的：从胎鼠侧脑室中分离神经干细胞，并对其进行培养及鉴定。方法采用无血清培养基分离培养来自胎鼠侧脑室的神经干细胞，并诱导其分化，用免疫荧光细胞化学的方法检测BrdU、Nestin和SOX2以及神经元标记物TuJ1的表达。结果胎鼠的侧脑室能够分离培养出具有增殖能力的神经球，呈Nestin和SOX2阳性，分化诱导后产生TuJ1阳性的神经元。结论胎鼠的侧脑室能够分离培养出神经干细胞，并能够在体外增殖、诱导分化为神经元。
목적：종태서측뇌실중분리신경간세포，병대기진행배양급감정。방법채용무혈청배양기분리배양래자태서측뇌실적신경간세포，병유도기분화，용면역형광세포화학적방법검측BrdU、Nestin화SOX2이급신경원표기물TuJ1적표체。결과태서적측뇌실능구분리배양출구유증식능력적신경구，정Nestin화SOX2양성，분화유도후산생TuJ1양성적신경원。결론태서적측뇌실능구분리배양출신경간세포，병능구재체외증식、유도분화위신경원。
Objective To culture and identify the neural stem cells (NSCs) in lateral ventricle of fetal mice. Methods NSCs were isolated from the lateral ventricle of fetal mice and cultured in serum-free medium. Fluorescence immunocytochemistry was carried out to examine the expression BrdU, the NSC markers Nestin and SOX2 and neuronal marker TuJ1. Results NSCs possessing proliferative capacities were obtained from the lateral ventricle of fetal mice. The cells were positive for NSC markers Nestin and SOX2, which were able to differentiate into TuJ1-positive neurons. Conclusion The cells isolated from the lateral ventricle of fetal mice are NSCs, which furthermore differentiated into neurons.